Endothelial-derived APT1-mediated macrophage-endothelial cell interactions participate in the development of atherosclerosis by regulating the Ras/MAPK signaling pathway
Journal article
Wang, Xinghua, Cheng, Lijun, Fu, Huaying, Chan, Calista Zhuo Yi, Tse, Gary, Liu, Tong and Li, Guangping 2022. Endothelial-derived APT1-mediated macrophage-endothelial cell interactions participate in the development of atherosclerosis by regulating the Ras/MAPK signaling pathway. Life. 12 (4), p. 551. https://doi.org/10.3390/life12040551
Authors | Wang, Xinghua, Cheng, Lijun, Fu, Huaying, Chan, Calista Zhuo Yi, Tse, Gary, Liu, Tong and Li, Guangping |
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Abstract | Acyl-protein thioesterase 1 (APT1) can affect H-Ras localization and function by promoting its depalmitoylation. However, relatively little attention has been paid to the effects of APT1 on H-Ras in the cardiovascular system. In this study, we revealed its roles in atherosclerosis development using oxidative low-density lipoprotein (ox-LDL)-induced endothelial dysfunction models and a Western diet-induced ApoE−/− mouse model. The results showed that APT1 expression was up-regulated, while that of miR-138-5p (miR-138) was down-regulated (p < 0.05) in this model. In the meantime, APT1 and H-Ras were translocated from the cytoplasm to the plasma membrane. Bioinformatic analysis and double fluorescence identified miR-138 as the upstream regulator of APT1. APT1 knockdown regulated H-Ras localization and expression, which subsequently affected the MAPK signaling pathway and the expression of its downstream factors. Further research indicated that human umbilical vein endothelial cells (HUVECs)-derived biogenic nanoparticles (BiNPs), hBPs secretion, and RNA expression of hBP-loaded APT1 were increased (p < 0.05) in the ox-LDL induced endothelial dysfunction model. Meanwhile, the HUVECs-derived APT1 could further affect macrophage function through hBP transportation. Altogether, this study demonstrated that the miR-138-APT1 axis may be partially responsible for atherosclerosis development by regulating the H-Ras-MAPK signaling pathway and hBP transportation. The results also shed novel insight on the underlying mechanisms of, and identify potential diagnostic and therapeutic targets for, atherosclerotic cardiovascular diseases in the future. |
Keywords | Atherosclerosis; APT1; miR-138-5p; Extracellular vesicles; Ras/MAPK signaling pathway |
Year | 2022 |
Journal | Life |
Journal citation | 12 (4), p. 551 |
Publisher | MDPI AG |
ISSN | 2075-1729 |
Digital Object Identifier (DOI) | https://doi.org/10.3390/life12040551 |
Official URL | https://www.mdpi.com/2075-1729/12/4/551 |
Funder | Natural Science Foundation of Tianjin City |
Publication dates | |
Online | 07 Apr 2022 |
Publication process dates | |
Accepted | 30 Mar 2022 |
Deposited | 07 Aug 2024 |
Publisher's version | License File Access Level Open |
Output status | Published |
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https://repository.canterbury.ac.uk/item/989w1/endothelial-derived-apt1-mediated-macrophage-endothelial-cell-interactions-participate-in-the-development-of-atherosclerosis-by-regulating-the-ras-mapk-signaling-pathway
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