Cellulase stimulation during biodegradation of lignocellulosic residues at increased biomass loading

Journal article


Pamidipati, S. and Ahmed, A. 2018. Cellulase stimulation during biodegradation of lignocellulosic residues at increased biomass loading. Biocatalysis and Biotransformation. https://doi.org/10.1080/10242422.2018.1508284
AuthorsPamidipati, S. and Ahmed, A.
Abstract

Microbial degradation of lignocellulosic biomass is primarily affected by the composition and structure of biomass, as well as enzyme activities that are influenced by the presence of in-process degradation products.

This study focuses on the latter, and demonstrates that cellulase activity of Neurospora discreta is stimulated in the presence of in-process soluble lignin degradation products. Two types of biomass - cocopeat and sugarcane bagasse, with contrasting lignin content and cellulose structure were tested at two biomass loadings each. At the higher biomass loading, cocopeat showed the highest amount of hydrolyzed cellulose and cellulase activity, despite its low cellulose content and recalcitrant cellulose structure. A strong positive correlation was revealed between the amount of in-process degraded lignin and cellulase activity, indicating a stimulatory effect on cellulase, which contradicts most previous literature. Furthermore, the causal relationship between the amount of degraded lignin and cellulase activity was established in a model system of commercial cellulase and standard soluble lignin.

This work could pave the way for using biomass loading as a process lever to enhance cellulose hydrolysis in microbial conversion of lignocellulosic biomass.

KeywordsCellulase stimulation; cellulose hydrolysis; cocopeat; lignin degradation products; Neurospora discreta; sugarcane bagasse
Year2018
JournalBiocatalysis and Biotransformation
PublisherTaylor & Francis
ISSN1024-2422
Digital Object Identifier (DOI)https://doi.org/10.1080/10242422.2018.1508284
Publication dates
Online11 Nov 2018
Publication process dates
Deposited02 Jul 2019
Accepted29 Jul 2018
Accepted author manuscript
Output statusPublished
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https://repository.canterbury.ac.uk/item/89011/cellulase-stimulation-during-biodegradation-of-lignocellulosic-residues-at-increased-biomass-loading

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