Identification of optimal assisted aspiration conditions of oocytes for use in porcine in vitro maturation: a re-evaluation of the relationship between the cumulus oocyte complex and oocyte quality

Journal article


Silvestri, S., Rathje, C.C., Harvey, S.C., Gould, R.L., Walling, G.A., Ellis, P.J.I., Harvey, K.E. and Griffin D.K. 2020. Identification of optimal assisted aspiration conditions of oocytes for use in porcine in vitro maturation: a re-evaluation of the relationship between the cumulus oocyte complex and oocyte quality. Veterinary Medicine and Science.
AuthorsSilvestri, S., Rathje, C.C., Harvey, S.C., Gould, R.L., Walling, G.A., Ellis, P.J.I., Harvey, K.E. and Griffin D.K.
Abstract

The quality of porcine oocytes for use in IVF is commonly graded according to the number of layers of cumulus cells surrounding the oocyte; together these form the cumulus oocyte complex (COC). At least three compact layers of cumulus cells is regarded as important for efficient IVP. To test this, oocytes were scored according to cumulus investment, with grade A representing COCs with three or more cumulus layers including granulosa cell-cumulus oocyte complexes, grade B those with an intact corona radiata surrounded by another layer of cumulus cells and grades C and D representing COCs with lower cumulus cell investment. These oocytes were then monitored for in vitro maturation (IVM), as assessed by tubulin immunostaining for meiotic progression, the development of a cortical granule ring, and by glutathione levels. Results indicate that grading correlates closely with nuclear maturation and cytoplasmic maturation, suggesting that grading oocytes by cumulus investment is a reliable method to predict IVM success. Importantly, Grade A and B oocytes showed no significant differences in any measure and hence using a cut-off of two or more cumulus cell layers may be optimal. We also determined the effect of assisted aspiration for oocyte retrieval, comparing the effect of needle size and applied pressure on the retrieval rate. These data indicated that both variables affected oocyte recovery rates and the quality of recovered oocytes. In combination, these experiments indicate that grade A and B oocytes have a similar developmental potential and that the recovery of oocytes of these grades is maximised by use of an 18-gauge needle and 50mmHg aspiration pressure.

KeywordsOocyte; Pig; In vitro production; Morphology
Year2020
JournalVeterinary Medicine and Science
PublisherWiley
ISSN2053-1095
Publication process dates
Deposited24 Sep 2020
Accepted16 Sep 2020
Accepted author manuscript
License
Output statusIn press
References

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Groenen, M., Archibald, A., Uenishi, H., Tuggle, C., Takeuchi, Y., Rothschild, M., Rogel-Gaillard, C., Park, C., Milan, D., Megens, H., Li, S., Larkin, D., Kim, H., Frantz, L., Caccamo, M., Ahn, H., Aken, B., Anselmo, A., Anthon, C., Auvil, L., Badaoui, B., Beattie, C., Bendixen, C., Berman, D., Blecha, F., Blomberg, J., Bolund, L., Bosse, M., Botti, S., Bujie, Z., Bystrom, M., Capitanu, B., Carvalho-Silva, D., Chardon, P., Chen, C., Cheng, R., Choi, S., Chow, W., Clark, R., Clee, C., Crooijmans, R., Dawson, H., Dehais, P., De Sapio, F., Dibbits, B., Drou, N., Du, Z., Eversole, K., Fadista, J., Fairley, S., Faraut, T., Faulkner, G., Fowler, K., Fredholm, M., Fritz, E., Gilbert, J., Giuffra, E., Gorodkin, J., Griffin, D., Harrow, J., Hayward, A., Howe, K., Hu, Z., Humphray, S., Hunt, T., Hornshøj, H., Jeon, J., Jern, P., Jones, M., Jurka, J., Kanamori, H., Kapetanovic, R., Kim, J., Kim, J., Kim, K., Kim, T., Larson, G., Lee, K., Lee, K., Leggett, R., Lewin, H., Li, Y., Liu, W., Loveland, J., Lu, Y., Lunney, J., Ma, J., Madsen, O., Mann, K., Matthews, L., McLaren, S., Morozumi, T., Murtaugh, M., Narayan, J., Truong Nguyen, D., Ni, P., Oh, S., Onteru, S., Panitz, F., Park, E., Park, H., Pascal, G., Paudel, Y., Perez-Enciso, M., Ramirez-Gonzalez, R., Reecy, J., Rodriguez-Zas, S., Rohrer, G., Rund, L., Sang, Y., Schachtschneider, K., Schraiber, J., Schwartz, J., Scobie, L., Scott, C., Searle, S., Servin, B., Southey, B., Sperber, G., Stadler, P., Sweedler, J., Tafer, H., Thomsen, B., Wali, R., Wang, J., Wang, J., White, S., Xu, X., Yerle, M., Zhang, G., Zhang, J., Zhang, J., Zhao, S., Rogers, J., Churcher, C. and Schook, L. 2012. Analyses of pig genomes provide insight into porcine demography and evolution. Nature. 491 (7424), pp. 393-398. https://doi.org/10.1038/nature11622
Comparative genomics in chicken and Pekin duck using FISH mapping and microarray analysis
Skinner, B., Robertson, L., Tempest, H., Langley, E., Ioannou, D., Fowler, K., Crooijmans, R., Hall, A., Griffin, D. and Völker, M. 2009. Comparative genomics in chicken and Pekin duck using FISH mapping and microarray analysis. BMC Genomics. 10 (1), p. 357. https://doi.org/10.1186/1471-2164-10-357
Widespread genomic incompatibilities in Caenorhabditis elegans
Snoek, L., Orbidans, H., Stastna, J., Aartse, A., Rodriguez, M., Riksen, J., Kammenga, J. and Harvey, S. 2014. Widespread genomic incompatibilities in Caenorhabditis elegans. G3. 4 (10), pp. 1813-1823. https://doi.org/10.1534/g3.114.013151
Highly polygenic variation in environmental perception determines dauer larvae formation in growing populations of Caenorhabditis elegans
Green, J., Stastna, J., Orbidans, H. and Harvey, S. 2014. Highly polygenic variation in environmental perception determines dauer larvae formation in growing populations of Caenorhabditis elegans. PLoS ONE. 9 (11), p. e112830. https://doi.org/10.1371/journal.pone.0112830
Genetic mapping of variation in dauer larvae development in growing populations of Caenorhabditis elegans
Green, J., Snoek, L., Kammenga, J. and Harvey, S. 2013. Genetic mapping of variation in dauer larvae development in growing populations of Caenorhabditis elegans. Heredity. https://doi.org/10.1038/hdy.2013.50
Genome wide analysis reveals single nucleotide polymorphisms associated with fatness and putative novel copy number variants in three pig breeds
Fowler, K., Pong-Wong, R., Bauer, J., Clemente, E., Reitter, C., Affara, N., Waite, S., Walling, G. and Griffin, D. 2013. Genome wide analysis reveals single nucleotide polymorphisms associated with fatness and putative novel copy number variants in three pig breeds. BMC Genomics. 14 (1), p. 784. https://doi.org/10.1186/1471-2164-14-784
Caenorhabditis elegans dauer larvae development in growing populations.
Green, J. and Harvey, S. 2012. Caenorhabditis elegans dauer larvae development in growing populations. Nematology. 14 (2), pp. 165-173. https://doi.org/10.1163/138855411x584115
A distributed systems approach to undergraduate assessment
Bertolo-Pardo, E. and Harvey, S. 2011. A distributed systems approach to undergraduate assessment.
Enhancing the teaching-research nexus in the undergraduate curriculum through assessment
Bertolo-Pardo, E., Capelo, J., Harvey, S. and Lodeiro, C. 2012. Enhancing the teaching-research nexus in the undergraduate curriculum through assessment.
All eggs are not equal: the maternal environment affects progeny reproduction and developmental fate in Caenorhabditis elegans
Harvey, S. and Orbidans, H. 2011. All eggs are not equal: the maternal environment affects progeny reproduction and developmental fate in Caenorhabditis elegans. PLoS ONE. 6 (10), p. e25840. https://doi.org/10.1371/journal.pone.0025840
Thermal stress responses in Caenorhabditis elegans: reproductive effects, natural variation and the costs of hormesis
Harvey, S. 2010. Thermal stress responses in Caenorhabditis elegans: reproductive effects, natural variation and the costs of hormesis.
Population growth and dauer larva development in Caenorhabditis elegans
Green, J. and Harvey, S. 2010. Population growth and dauer larva development in Caenorhabditis elegans.
Towards modelling the ecology of Caernorhabditis elegans: dauer larvae development in growing populations
Green, J. and Harvey, S. 2011. Towards modelling the ecology of Caernorhabditis elegans: dauer larvae development in growing populations.
Quantitative genetic analysis of life-history traits of Caenorhabditis elegans in stressful environments
Harvey, S., Shorto, A. and Viney, M. 2008. Quantitative genetic analysis of life-history traits of Caenorhabditis elegans in stressful environments. BMC Evolutionary Biology. 8 (1), p. 15. https://doi.org/10.1186/1471-2148-8-15
Heterogeneity in the distribution of Strongyloides ratti infective stages among the faecal pellets of rats
Harvey, S., Paterson, S. and Viney, M. 1999. Heterogeneity in the distribution of Strongyloides ratti infective stages among the faecal pellets of rats. Parasitology. 119 (2), pp. 227-235.
Sex determination in the parasitic nematode Strongyloides ratti
Harvey, S. and Viney, M. 2001. Sex determination in the parasitic nematode Strongyloides ratti. Genetics. 158, pp. 1527-1533.
Identification of putative sex chromosomes in the blue tilapia, Oreochromis aureus, through synaptonemal complex and FISH analysis
Campos-Ramos, R., Harvey, S., Masabanda, J., Carrasco, L., Griffin, D., McAndrew, B., Bromage, N. and Penman, D. 2001. Identification of putative sex chromosomes in the blue tilapia, Oreochromis aureus, through synaptonemal complex and FISH analysis. Genetica. 111 (1/3), pp. 143-153. https://doi.org/10.1023/A:1013707818534
Karyotype analysis of Oreochromis mortimeri (Trewavas) and Sarotherodon melanotheron (Ruppell)
Harvey, S., Powell, S., Kennedy, D., McAndrew, B. and Penman, D. 2002. Karyotype analysis of Oreochromis mortimeri (Trewavas) and Sarotherodon melanotheron (Ruppell). Aquaculture Research. 33 (5), pp. 339-342. https://doi.org/10.1046/j.1365-2109.2002.00678.x
Molecular-cytogenetic analysis reveals sequence differences between the sex chromosomes of Oreochromis niloticus: evidence for an early stage of sex-chromosome differentiation
Harvey, S., Masabanda, J., Carrasco, L., Bromage, N., Penman, D. and Griffin, D. 2002. Molecular-cytogenetic analysis reveals sequence differences between the sex chromosomes of Oreochromis niloticus: evidence for an early stage of sex-chromosome differentiation. Cytogenetic and Genome Research. 97 (1-2), pp. 76-80. https://doi.org/10.1159/000064036
Karyotype evolution in tilapia: mitotic and meiotic chromosome analysis of Oreochromis Karongae and O. Niloticus × O. Karongae hybrids
Harvey, S., Campos-Ramos, R., Kennedy, D., Ezaz, M., Bromage, N., Griffin, D. and Penman, D. 2002. Karyotype evolution in tilapia: mitotic and meiotic chromosome analysis of Oreochromis Karongae and O. Niloticus × O. Karongae hybrids. Genetica. 115 (2), pp. 169-177. https://doi.org/10.1023/A:1020190918431
Early origins of the X and Y chromosomes: lessons from tilapia
Griffin, D., Harvey, S., Campos-Ramos, R., Ayling, L., Bromage, N., Masabanda, J. and Penman, D. 2002. Early origins of the X and Y chromosomes: lessons from tilapia. Cytogenetic and Genome Research. 99 (1-4), pp. 157-163. https://doi.org/10.1159/000071588
An investigation of sex determination in the Mozambique tilapia, Oreochromis mossambicus, using synaptonemal complex analysis, FISH, sex reversal and gynogenesis
Campos-Ramos, R., Harvey, S., McAndrew, B. and Penman, D. 2003. An investigation of sex determination in the Mozambique tilapia, Oreochromis mossambicus, using synaptonemal complex analysis, FISH, sex reversal and gynogenesis. Aquaculture. 221 (1-4), pp. 125-140. https://doi.org/10.1016/S0044-8486(03)00072-3
Physical mapping of the brain and ovarian aromatase genes in the Nile tilapia, Oreochromis niloticus, by fluorescence in situ hybridization
Harvey, S., Kwon, J. and Penman, D. 2003. Physical mapping of the brain and ovarian aromatase genes in the Nile tilapia, Oreochromis niloticus, by fluorescence in situ hybridization. Animal Genetics. 34 (1), pp. 62-64. https://doi.org/10.1046/j.1365-2052.2003.00941.x
Analysis of repetitive DNA sequences in the sex chromosomes of Oreochromis niloticus
Harvey, S., Boonphakdee, C., Campos-Ramos, R., Ezaz, M., Griffin, D., Bromage, N. and Penman, D. 2003. Analysis of repetitive DNA sequences in the sex chromosomes of Oreochromis niloticus. Cytogenetic and Genome Research. 101 (3-4), pp. 314-319. https://doi.org/10.1159/000074355
Isolation and physical mapping of sex-linked AFLP markers in Nile tilapia (Oreochromis niloticus L.)
Ezaz, M., Harvey, S., Boonphakdee, C., Teale, A., McAndrew, B. and Penman, D. 2004. Isolation and physical mapping of sex-linked AFLP markers in Nile tilapia (Oreochromis niloticus L.). Marine Biotechnology. 6 (5), pp. 435-445. https://doi.org/10.1007/s10126-004-3004-6
Thermal variation reveals natural variation between isolates of Caenorhabditis elegans
Harvey, S. and Viney, M. 2007. Thermal variation reveals natural variation between isolates of Caenorhabditis elegans. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution. 308B (4), pp. 409-416. https://doi.org/10.1002/jez.b.21161
Sex-specific differences in the synaptonemal complex in the genus Oreochromis (Cichlidae)
Campos-Ramos, R., Harvey, S. and Penman, D. 2009. Sex-specific differences in the synaptonemal complex in the genus Oreochromis (Cichlidae). Genetica. 135 (3), pp. 325-332. https://doi.org/10.1007/s10709-008-9280-8
Natural variation in gene expression in the early development of dauer larvae of Caenorhabditis elegans
Harvey, S., Barker, G., Shorto, A. and Viney, M. 2009. Natural variation in gene expression in the early development of dauer larvae of Caenorhabditis elegans. BMC Genomics. 10 (1), p. 325. https://doi.org/10.1186/1471-2164-10-325
Non-dauer larval dispersal in Caenorhabditis elegans
Harvey, S. 2009. Non-dauer larval dispersal in Caenorhabditis elegans. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution. 312B (3), pp. 224-230. https://doi.org/10.1002/jez.b.21287
The control of morph development in the parasitic nematode Strongyloides ratti
Harvey, S., Gemmill, A., Read, A. and Viney, M. 2000. The control of morph development in the parasitic nematode Strongyloides ratti. Proceedings of the Royal Society B: Biological Sciences. 267 (1457), pp. 2057-2063.