The role of apoptosis in cryopreserved animal oocytes and embryos

Journal article


Vining, Lucy May, Zak, Louisa J, Harvey, Simon Crawford and Harvey, K. 2021. The role of apoptosis in cryopreserved animal oocytes and embryos. Theriogenology. 173, pp. 93-101. https://doi.org/S0093-691X(21)00242-9
AuthorsVining, Lucy May, Zak, Louisa J, Harvey, Simon Crawford and Harvey, K.
AbstractCryopreservation of both gametes and embryos, both for storage and for the preservation of their developmental capacity is a critical aspect of assisted reproductive technology. The survival of reproductive material following cryopreservation protocols is not only vital to clinical applications in the human in vitro fertilisation clinic, but is also important in the in vitro production of livestock embryos. The ability to routinely cryopreserve oocytes and embryos of livestock species has the potential to improve animal welfare, reduce environmental impact, and reduce the associated costs for breeding companies through the reduction of live animal transportation. Unfortunately, frozen oocytes and embryos are regularly documented to contain a higher proportion of apoptotic cells compared to their non-frozen counterparts, with freezing procedures thought to trigger apoptotic pathways of cell death. Comparisons between frozen and non-frozen samples also show changes in the gene expression of apoptotic factors such as Bcl-2 and Bax in response to cryopreservation. Apoptotic inhibition has the potential to improve cryosurvival, and how to achieve this is subject to debate. Here, we review how exposure to low temperatures during cryopreservation may be responsible for the abnormal activation of apoptotic pathways in mammalian oocytes and embryos, and discuss the ways in which they can be influenced to improve cryopreservation protocols, particularly in agriculturally important species. [Abstract copyright: Copyright © 2021 Elsevier Inc. All rights reserved.]
KeywordsApoptosis; Cryopreservation; Bovine; Cell death; Porcine
Year2021
JournalTheriogenology
Journal citation173, pp. 93-101
PublisherElsevier
ISSN1879-3231
Digital Object Identifier (DOI)https://doi.org/S0093-691X(21)00242-9
https://doi.org/10.1016/j.theriogenology.2021.07.017
Publication dates
Online26 Jul 2021
Publication process dates
Deposited13 Sep 2021
Accepted25 Jul 2021
Accepted author manuscript
File Access Level
Restricted
Output statusIn press
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Publications router: Date 2021-07-25 of type 'accepted_date' included in notification.
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Publications router: Date 2021-03-02 of type 'received' included in notification
Publications router: Date 2021-07-24 of type 'revised' included in notification
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