Morphokinetic profiling suggests that rapid first cleavage division accurately predicts the chances of blastulation in pig In vitro produced embryos

Journal article

Hillyear, Lucy M., Zak, L., Beckitt, T., Griffin, D., Harvey, Simon C. and Harvey, K. 2024. Morphokinetic profiling suggests that rapid first cleavage division accurately predicts the chances of blastulation in pig In vitro produced embryos. Animals. 14 (5), p. 783. https://doi.org/10.3390/ani14050783
AuthorsHillyear, Lucy M., Zak, L., Beckitt, T., Griffin, D., Harvey, Simon C. and Harvey, K.
AbstractThe study of pig preimplantation embryo development has several potential uses: from agriculture to the production of medically relevant genetically modified organisms and from rare breed conservation to acting as a physiologically relevant model for progressing human and other (e.g., endangered) species’ in vitro fertilisation technology. Despite this, barriers to the widespread adoption of pig embryo in vitro production include lipid-laden cells that are hard to visualise, slow adoption of contemporary technologies such as the use of time-lapse incubators or artificial intelligence, poor blastulation and high polyspermy rates. Here, we employ a commercially available time-lapse incubator to provide a comprehensive overview of the morphokinetics of pig preimplantation development for the first time. We tested the hypotheses that (a) there are differences in developmental timings between blastulating and non-blastulating embryos and (b) embryo developmental morphokinetic features can be used to predict the likelihood of blastulation. The abattoir-derived oocytes fertilised by commercial extended semen produced presumptive zygotes were split into two groups: cavitating/blastulating 144 h post gamete co-incubation and those that were not. The blastulating group reached the 2-cell and morula stages significantly earlier, and the time taken to reach the 2-cell stage was identified to be a predictive marker for blastocyst formation. Reverse cleavage was also associated with poor blastulation. These data demonstrate the potential of morphokinetic analysis in automating and upscaling pig in vitro production through effective embryo selection.
KeywordsBlastcyst; Morphokinetics; Pig; Predictive parameters; Time-lapse
Year2024
JournalAnimals
Journal citation14 (5), p. 783
PublisherMDPI
ISSN2076-2615
Digital Object Identifier (DOI)https://doi.org/10.3390/ani14050783
Official URLhttps://www.mdpi.com/2076-2615/14/5/783
Publication dates
Online02 Mar 2024
Publication process dates
Accepted28 Feb 2024
Deposited14 Mar 2024
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Open
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