Morphokinetic profiling suggests that rapid first cleavage division accurately predicts the chances of blastulation in pig In vitro produced embryos
Journal article
Hillyear, Lucy M., Zak, L., Beckitt, T., Griffin, D., Harvey, Simon C. and Harvey, K. 2024. Morphokinetic profiling suggests that rapid first cleavage division accurately predicts the chances of blastulation in pig In vitro produced embryos. Animals. 14 (5), p. 783. https://doi.org/10.3390/ani14050783
Authors | Hillyear, Lucy M., Zak, L., Beckitt, T., Griffin, D., Harvey, Simon C. and Harvey, K. |
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Abstract | The study of pig preimplantation embryo development has several potential uses: from agriculture to the production of medically relevant genetically modified organisms and from rare breed conservation to acting as a physiologically relevant model for progressing human and other (e.g., endangered) species’ in vitro fertilisation technology. Despite this, barriers to the widespread adoption of pig embryo in vitro production include lipid-laden cells that are hard to visualise, slow adoption of contemporary technologies such as the use of time-lapse incubators or artificial intelligence, poor blastulation and high polyspermy rates. Here, we employ a commercially available time-lapse incubator to provide a comprehensive overview of the morphokinetics of pig preimplantation development for the first time. We tested the hypotheses that (a) there are differences in developmental timings between blastulating and non-blastulating embryos and (b) embryo developmental morphokinetic features can be used to predict the likelihood of blastulation. The abattoir-derived oocytes fertilised by commercial extended semen produced presumptive zygotes were split into two groups: cavitating/blastulating 144 h post gamete co-incubation and those that were not. The blastulating group reached the 2-cell and morula stages significantly earlier, and the time taken to reach the 2-cell stage was identified to be a predictive marker for blastocyst formation. Reverse cleavage was also associated with poor blastulation. These data demonstrate the potential of morphokinetic analysis in automating and upscaling pig in vitro production through effective embryo selection. |
Keywords | Blastcyst; Morphokinetics; Pig; Predictive parameters; Time-lapse |
Year | 2024 |
Journal | Animals |
Journal citation | 14 (5), p. 783 |
Publisher | MDPI |
ISSN | 2076-2615 |
Digital Object Identifier (DOI) | https://doi.org/10.3390/ani14050783 |
Official URL | https://www.mdpi.com/2076-2615/14/5/783 |
Publication dates | |
Online | 02 Mar 2024 |
Publication process dates | |
Accepted | 28 Feb 2024 |
Deposited | 14 Mar 2024 |
Publisher's version | License File Access Level Open |
Output status | Published |
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https://repository.canterbury.ac.uk/item/975w0/morphokinetic-profiling-suggests-that-rapid-first-cleavage-division-accurately-predicts-the-chances-of-blastulation-in-pig-in-vitro-produced-embryos
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