Cryopreservation produces limited long-term effects on the nematode Caenorhabditis elegans

Journal article


Stastna, J., Yiapanas, A., Mandawala, D., Fowler, K. and Harvey, S. 2019. Cryopreservation produces limited long-term effects on the nematode Caenorhabditis elegans . Cryobiology . https://doi.org/0.​1016/​j.​cryobiol.​2019.​11.​039
AuthorsStastna, J., Yiapanas, A., Mandawala, D., Fowler, K. and Harvey, S.
Abstract

Cryopreservation, the freezing and later warming of biological samples with minimal loss of viability, is important in many scientific disciplines. For some applications, particularly those where there is limited available material, it is critical to ensure the maximal survival rates of cryopreserved materials. Most of the challenges encountered with such techniques take place after the warming process where cryodamage affects cell viability and future development. Here we have used the nematode Caenorhabditis elegans to investigate the effects of cryodamage caused by slow-freezing. We find that freezing results in the death of some worms, with an approximately 40% reduction in the number of worms that develop in the frozen populations, but that the effects on worms that survive are limited. For example, there are no differences in the lifetime fecundity or in lifespan between frozen and control worms, although early fecundity and body size was reduced in frozen worms. Similarly, analyses of body wall muscle structure and of pharyngeal function indicates that muscle development and function are not significantly affected by freezing. We do however determine that freezing increases the rates of matricidal hatching, where progeny hatch within the mother. Overall, these results indicate that, for worms that survive, cryopreservation produces limited long-term effects, but do indicate that some phenotypes could be used in further analyses of the cellular damage induced by cryopreservation.

KeywordsC. elegans; Freezing biological samples; Cryopreservation; Cryodamage
Year2019
JournalCryobiology
PublisherElsevier
ISSN0011-2240
Digital Object Identifier (DOI)https://doi.org/0.​1016/​j.​cryobiol.​2019.​11.​039
Official URLhttps://doi.org/10.1016/j.cryobiol.2019.11.039
Publication dates
Online23 Nov 2019
Publication process dates
Accepted22 Nov 2019
Deposited29 Nov 2019
Accepted author manuscript
License
File Access Level
Open
Output statusPublished
Permalink -

https://repository.canterbury.ac.uk/item/8917q/cryopreservation-produces-limited-long-term-effects-on-the-nematode-caenorhabditis-elegans

Restricted files

Accepted author manuscript

  • 10
    total views
  • 0
    total downloads
  • 0
    views this month
  • 0
    downloads this month

Export as

Related outputs

Identification of optimal assisted aspiration conditions of oocytes for use in porcine in vitro maturation: a re-evaluation of the relationship between the cumulus oocyte complex and oocyte quality
Silvestri, S., Rathje, C.C., Harvey, S.C., Gould, R.L., Walling, G.A., Ellis, P.J.I., Harvey, K.E. and Griffin D.K. 2020. Identification of optimal assisted aspiration conditions of oocytes for use in porcine in vitro maturation: a re-evaluation of the relationship between the cumulus oocyte complex and oocyte quality. Veterinary Medicine and Science.
Eukaryotic response to hypothermia in relation to integrated stress responses
Adjirackor, N. A., Harvey, K. E. and Harvey, S. C. 2020. Eukaryotic response to hypothermia in relation to integrated stress responses. Cell Stress & Chaperones.
Genetic variation in complex traits in transgenic α-synuclein strains of Caenorhabditis elegans
Wang, Y., van Sluijs, L., Nie, Y., Sterken, M.G., Harvey, S. and Kammenga, J.E. 2020. Genetic variation in complex traits in transgenic α-synuclein strains of Caenorhabditis elegans. Genes. 11 (7), p. 778. https://doi.org/10.3390/genes11070778
Genetic background modifies phenotypic and transcriptional responses in a C. elegans model of α-synuclein toxicity
Wang, Y., Snoek, B., Sterken, M., Riksen, J., Stastna, J., Kammenga, J. and Harvey, S. 2019. Genetic background modifies phenotypic and transcriptional responses in a C. elegans model of α-synuclein toxicity. BMC Genomics. 20 (1), p. 232. https://doi.org/10.1186/s12864-019-5597-1
The role of chromosome segregation and nuclear organisation in human subfertility
Fowler, K., Mandawala, A. and Griffin, D. 2019. The role of chromosome segregation and nuclear organisation in human subfertility. Biochemical Society Transactions. 47 (1), pp. 425-432. https://doi.org/10.1042/BST20180231
A multi-parent recombinant inbred line population of C. elegans allows identification of novel QTLs for complex life history traits
Snoek, L., Volkers, R., Petersen, C., Dirksen, P., Sterken, M., Nakad, R., Riksen, J., Ronsenstiel, P., Stastna, J., Braeckman, B., Harvey, S., Schulenburg, H., Kammenga, J. and Nijveen, H. 2019. A multi-parent recombinant inbred line population of C. elegans allows identification of novel QTLs for complex life history traits. BMC Biology. https://doi.org/10.1101/443135
Synthesis of gold functionalised nanoparticles with the eranthis hyemalis lectin and preliminary toxicological studies on caenorhabditis elegans
Djafari, J., McConnell, M., Santos, H., Capelo, J., Bertolo-Pardo, E., Harvey, S., Lodeiro, C. and Fernández-Lodeiro, J. 2018. Synthesis of gold functionalised nanoparticles with the eranthis hyemalis lectin and preliminary toxicological studies on caenorhabditis elegans. Materials. 11 (8). https://doi.org/10.3390/ma11081363
The production of pig preimplantation embryos in vitro: current progress and future prospects
Fowler, K., Mandawala, A., Griffin, D., Walling, G. and Harvey, S. 2018. The production of pig preimplantation embryos in vitro: current progress and future prospects. Reproductive Biology. 18 (3), pp. 203-211. https://doi.org/10.1016/j.repbio.2018.07.001
Canine recommended breed weight ranges are not a good predictor of an ideal body condition score
Smith, E., Davis, K., Sulsh, L., Harvey, S., Fowler, K., Canterbury Christ Church University and Portland Road Veterinary Surgery 2018. Canine recommended breed weight ranges are not a good predictor of an ideal body condition score. Journal Of Animal Physiology And Nutrition. 102 (4), pp. 1088-1090. https://doi.org/10.1111/jpn.12919
Mutation independently affects reproductive traits and dauer larvae development in mutation accumulation lines of Caenorhabditis elegans
Hills, A., Green, J. and Harvey, S. 2017. Mutation independently affects reproductive traits and dauer larvae development in mutation accumulation lines of Caenorhabditis elegans. Development genes and evolution. 227 (6), pp. 411-414. https://doi.org/10.1007/s00427-017-0596-1
Consensus statement on placebo effects in sports and exercise: the need for conceptual clarity, methodological rigour, and the elucidation of neurobiological mechanisms.
Beedie, C., Hurst, P., Coleman, D., Foad, A., Benedetti, F., Cohen, E., Davis, A., Elseworth-Edelsten, C., Flowers, E., Roelands, B., Hettinga, F., Raglin, J., Szabo, A., Camerone, E., Barbiani, D., Lane, A., Lindheimer, J., Schiphof-Godart, l. and Harvey, S. 2018. Consensus statement on placebo effects in sports and exercise: the need for conceptual clarity, methodological rigour, and the elucidation of neurobiological mechanisms. European Journal of Sport Science. https://doi.org/10.1080/17461391.2018.1496144
Studying placebo effects in model organisms will help us understand them in humans
Harvey, S. and Beedie, C. 2017. Studying placebo effects in model organisms will help us understand them in humans. Biology Letters. 13 (11). https://doi.org/10.1098/rsbl.2017.0585
Genetic variation in neurodegenerative diseases and its accessibility in the model organism Caenorhabditis elegans
Wang, Y., Kammenga, J. and Harvey, S. 2017. Genetic variation in neurodegenerative diseases and its accessibility in the model organism Caenorhabditis elegans. Human Genomics. 11 (1). https://doi.org/10.1186/s40246-017-0108-4
Reimagining pheromone signalling in the model nematode Caenorhabditis elegans
Viney, M. and Harvey, S. 2017. Reimagining pheromone signalling in the model nematode Caenorhabditis elegans. PLoS Genetics. 13 (11), p. e1007046. https://doi.org/10.1371/journal.pgen.1007046
Upgrading short read animal genome assemblies to chromosome level using comparative genomics and a universal probe set
Damas, J., O'Connor, R., Farré, M., Lenis, V., Martell, H., Mandawala, A., Fowler, K., Joseph, S., Swain, M., Griffin, D. and Larkin, D. 2016. Upgrading short read animal genome assemblies to chromosome level using comparative genomics and a universal probe set. Genome Research.
Time-lapse embryo imaging and morphokinetic profiling: towards a general characterisation of embryogenesis
Mandawala, A., Harvey, S., Roy, T. and Fowler, K. 2016. Time-lapse embryo imaging and morphokinetic profiling: towards a general characterisation of embryogenesis. Animal Reproduction Science. 174, pp. 2-10. https://doi.org/10.1016/j.anireprosci.2016.09.015
Cryopreservation of animal oocytes and embryos: current progress and future prospects
Mandawala, A., Harvey, S., Roy, T. and Fowler, K. 2016. Cryopreservation of animal oocytes and embryos: current progress and future prospects. Theriogenology. 86 (7), pp. 1637-1644. https://doi.org/10.1016/j.theriogenology.2016.07.018
Expanding the view on the evolution of the nematode dauer signalling pathways: refinement through gene gain and pathway co-option
Gilabert, A., Curran, D., Harvey, S. and Wasmuth, J. 2016. Expanding the view on the evolution of the nematode dauer signalling pathways: refinement through gene gain and pathway co-option. BMC Genomics. 17, p. 476. https://doi.org/10.1186/s12864-016-2770-7
Multicolour detection of every chromosome as a means of detecting mosaicism and nuclear organisation in human embryonic nuclei
Turner, K., Fowler, K., Fonseka, G., Griffin, D. and Ioannou, D. 2016. Multicolour detection of every chromosome as a means of detecting mosaicism and nuclear organisation in human embryonic nuclei. Panminerva Medica.
Natural Variation in lifespan and stress responses in Caenorhabditis elegans
Stastna, J. 2016. Natural Variation in lifespan and stress responses in Caenorhabditis elegans. PhD Thesis Canterbury Christ Church University School of Human and Life Sciences
Winter Aconite (Eranthis hyemalis) Lectin as a cytotoxic effector in the lifecycle of Caenorhabditis elegans
McConnell, M., Lisgarten, D., Byrne, L., Harvey, S. and Bertolo-Pardo, E. 2015. Winter Aconite (Eranthis hyemalis) Lectin as a cytotoxic effector in the lifecycle of Caenorhabditis elegans. PeerJ. https://doi.org/10.7717/peerj.1206
[Avian cytogenetics goes functional] Third report on chicken genes and chromosomes 2015
Grffin, D., Romanov, M., O'Connor, R., Fowler, K. and Larkin, D. 2015. [Avian cytogenetics goes functional] Third report on chicken genes and chromosomes 2015. Cytogenetic and Genome Research. 145 (2), pp. 78-179. https://doi.org/10.1159/000430927
20th International Chromosome Conference (ICCXX)
Griffin, D., Fowler, K., Ellis, P. and Jackson, D. 2015. 20th International Chromosome Conference (ICCXX). Chromosome Research. https://doi.org/10.1007/s10577-014-9447-3
Genotype-dependent lifespan effects in peptone deprived Caenorhabditis elegans
Stastna, J., Snoek, L., Kammenga, J. and Harvey, S. 2015. Genotype-dependent lifespan effects in peptone deprived Caenorhabditis elegans. Scientific Reports. 5 (16259). https://doi.org/10.1038/srep16259
Reconstruction of gross avian genome structure, organization and evolution suggests that the chicken lineage most closely resembles the dinosaur avian ancestor
Romanov, M., Farré, M., Lithgow, P., Fowler, K., Skinner, B., O'Connor, R., Fonseka, G., Backström, N., Matsuda, Y., Nishida, C., Houde, P., Jarvis, E., Hanson, E., Burt, D., Larkin, D. and Griffin, D. 2014. Reconstruction of gross avian genome structure, organization and evolution suggests that the chicken lineage most closely resembles the dinosaur avian ancestor. BMC Genomics. 15 (1060). https://doi.org/10.1186/1471-2164-15-1060
Novel approach for deriving genome wide SNP analysis data from archived blood spots
Fowler, K., Reitter, C., Walling, G. and Griffin, D. 2012. Novel approach for deriving genome wide SNP analysis data from archived blood spots. BMC Research Notes. 5 (1), p. 503. https://doi.org/10.1186/1756-0500-5-503
Analyses of pig genomes provide insight into porcine demography and evolution
Groenen, M., Archibald, A., Uenishi, H., Tuggle, C., Takeuchi, Y., Rothschild, M., Rogel-Gaillard, C., Park, C., Milan, D., Megens, H., Li, S., Larkin, D., Kim, H., Frantz, L., Caccamo, M., Ahn, H., Aken, B., Anselmo, A., Anthon, C., Auvil, L., Badaoui, B., Beattie, C., Bendixen, C., Berman, D., Blecha, F., Blomberg, J., Bolund, L., Bosse, M., Botti, S., Bujie, Z., Bystrom, M., Capitanu, B., Carvalho-Silva, D., Chardon, P., Chen, C., Cheng, R., Choi, S., Chow, W., Clark, R., Clee, C., Crooijmans, R., Dawson, H., Dehais, P., De Sapio, F., Dibbits, B., Drou, N., Du, Z., Eversole, K., Fadista, J., Fairley, S., Faraut, T., Faulkner, G., Fowler, K., Fredholm, M., Fritz, E., Gilbert, J., Giuffra, E., Gorodkin, J., Griffin, D., Harrow, J., Hayward, A., Howe, K., Hu, Z., Humphray, S., Hunt, T., Hornshøj, H., Jeon, J., Jern, P., Jones, M., Jurka, J., Kanamori, H., Kapetanovic, R., Kim, J., Kim, J., Kim, K., Kim, T., Larson, G., Lee, K., Lee, K., Leggett, R., Lewin, H., Li, Y., Liu, W., Loveland, J., Lu, Y., Lunney, J., Ma, J., Madsen, O., Mann, K., Matthews, L., McLaren, S., Morozumi, T., Murtaugh, M., Narayan, J., Truong Nguyen, D., Ni, P., Oh, S., Onteru, S., Panitz, F., Park, E., Park, H., Pascal, G., Paudel, Y., Perez-Enciso, M., Ramirez-Gonzalez, R., Reecy, J., Rodriguez-Zas, S., Rohrer, G., Rund, L., Sang, Y., Schachtschneider, K., Schraiber, J., Schwartz, J., Scobie, L., Scott, C., Searle, S., Servin, B., Southey, B., Sperber, G., Stadler, P., Sweedler, J., Tafer, H., Thomsen, B., Wali, R., Wang, J., Wang, J., White, S., Xu, X., Yerle, M., Zhang, G., Zhang, J., Zhang, J., Zhao, S., Rogers, J., Churcher, C. and Schook, L. 2012. Analyses of pig genomes provide insight into porcine demography and evolution. Nature. 491 (7424), pp. 393-398. https://doi.org/10.1038/nature11622
Comparative genomics in chicken and Pekin duck using FISH mapping and microarray analysis
Skinner, B., Robertson, L., Tempest, H., Langley, E., Ioannou, D., Fowler, K., Crooijmans, R., Hall, A., Griffin, D. and Völker, M. 2009. Comparative genomics in chicken and Pekin duck using FISH mapping and microarray analysis. BMC Genomics. 10 (1), p. 357. https://doi.org/10.1186/1471-2164-10-357
Widespread genomic incompatibilities in Caenorhabditis elegans
Snoek, L., Orbidans, H., Stastna, J., Aartse, A., Rodriguez, M., Riksen, J., Kammenga, J. and Harvey, S. 2014. Widespread genomic incompatibilities in Caenorhabditis elegans. G3. 4 (10), pp. 1813-1823. https://doi.org/10.1534/g3.114.013151
Highly polygenic variation in environmental perception determines dauer larvae formation in growing populations of Caenorhabditis elegans
Green, J., Stastna, J., Orbidans, H. and Harvey, S. 2014. Highly polygenic variation in environmental perception determines dauer larvae formation in growing populations of Caenorhabditis elegans. PLoS ONE. 9 (11), p. e112830. https://doi.org/10.1371/journal.pone.0112830
Genetic mapping of variation in dauer larvae development in growing populations of Caenorhabditis elegans
Green, J., Snoek, L., Kammenga, J. and Harvey, S. 2013. Genetic mapping of variation in dauer larvae development in growing populations of Caenorhabditis elegans. Heredity. https://doi.org/10.1038/hdy.2013.50
Genome wide analysis reveals single nucleotide polymorphisms associated with fatness and putative novel copy number variants in three pig breeds
Fowler, K., Pong-Wong, R., Bauer, J., Clemente, E., Reitter, C., Affara, N., Waite, S., Walling, G. and Griffin, D. 2013. Genome wide analysis reveals single nucleotide polymorphisms associated with fatness and putative novel copy number variants in three pig breeds. BMC Genomics. 14 (1), p. 784. https://doi.org/10.1186/1471-2164-14-784
Caenorhabditis elegans dauer larvae development in growing populations.
Green, J. and Harvey, S. 2012. Caenorhabditis elegans dauer larvae development in growing populations. Nematology. 14 (2), pp. 165-173. https://doi.org/10.1163/138855411x584115
A distributed systems approach to undergraduate assessment
Bertolo-Pardo, E. and Harvey, S. 2011. A distributed systems approach to undergraduate assessment.
Enhancing the teaching-research nexus in the undergraduate curriculum through assessment
Bertolo-Pardo, E., Capelo, J., Harvey, S. and Lodeiro, C. 2012. Enhancing the teaching-research nexus in the undergraduate curriculum through assessment.
All eggs are not equal: the maternal environment affects progeny reproduction and developmental fate in Caenorhabditis elegans
Harvey, S. and Orbidans, H. 2011. All eggs are not equal: the maternal environment affects progeny reproduction and developmental fate in Caenorhabditis elegans. PLoS ONE. 6 (10), p. e25840. https://doi.org/10.1371/journal.pone.0025840
Thermal stress responses in Caenorhabditis elegans: reproductive effects, natural variation and the costs of hormesis
Harvey, S. 2010. Thermal stress responses in Caenorhabditis elegans: reproductive effects, natural variation and the costs of hormesis.
Population growth and dauer larva development in Caenorhabditis elegans
Green, J. and Harvey, S. 2010. Population growth and dauer larva development in Caenorhabditis elegans.
Towards modelling the ecology of Caernorhabditis elegans: dauer larvae development in growing populations
Green, J. and Harvey, S. 2011. Towards modelling the ecology of Caernorhabditis elegans: dauer larvae development in growing populations.
Quantitative genetic analysis of life-history traits of Caenorhabditis elegans in stressful environments
Harvey, S., Shorto, A. and Viney, M. 2008. Quantitative genetic analysis of life-history traits of Caenorhabditis elegans in stressful environments. BMC Evolutionary Biology. 8 (1), p. 15. https://doi.org/10.1186/1471-2148-8-15
Heterogeneity in the distribution of Strongyloides ratti infective stages among the faecal pellets of rats
Harvey, S., Paterson, S. and Viney, M. 1999. Heterogeneity in the distribution of Strongyloides ratti infective stages among the faecal pellets of rats. Parasitology. 119 (2), pp. 227-235.
Sex determination in the parasitic nematode Strongyloides ratti
Harvey, S. and Viney, M. 2001. Sex determination in the parasitic nematode Strongyloides ratti. Genetics. 158, pp. 1527-1533.
Identification of putative sex chromosomes in the blue tilapia, Oreochromis aureus, through synaptonemal complex and FISH analysis
Campos-Ramos, R., Harvey, S., Masabanda, J., Carrasco, L., Griffin, D., McAndrew, B., Bromage, N. and Penman, D. 2001. Identification of putative sex chromosomes in the blue tilapia, Oreochromis aureus, through synaptonemal complex and FISH analysis. Genetica. 111 (1/3), pp. 143-153. https://doi.org/10.1023/A:1013707818534
Karyotype analysis of Oreochromis mortimeri (Trewavas) and Sarotherodon melanotheron (Ruppell)
Harvey, S., Powell, S., Kennedy, D., McAndrew, B. and Penman, D. 2002. Karyotype analysis of Oreochromis mortimeri (Trewavas) and Sarotherodon melanotheron (Ruppell). Aquaculture Research. 33 (5), pp. 339-342. https://doi.org/10.1046/j.1365-2109.2002.00678.x
Molecular-cytogenetic analysis reveals sequence differences between the sex chromosomes of Oreochromis niloticus: evidence for an early stage of sex-chromosome differentiation
Harvey, S., Masabanda, J., Carrasco, L., Bromage, N., Penman, D. and Griffin, D. 2002. Molecular-cytogenetic analysis reveals sequence differences between the sex chromosomes of Oreochromis niloticus: evidence for an early stage of sex-chromosome differentiation. Cytogenetic and Genome Research. 97 (1-2), pp. 76-80. https://doi.org/10.1159/000064036
Karyotype evolution in tilapia: mitotic and meiotic chromosome analysis of Oreochromis Karongae and O. Niloticus × O. Karongae hybrids
Harvey, S., Campos-Ramos, R., Kennedy, D., Ezaz, M., Bromage, N., Griffin, D. and Penman, D. 2002. Karyotype evolution in tilapia: mitotic and meiotic chromosome analysis of Oreochromis Karongae and O. Niloticus × O. Karongae hybrids. Genetica. 115 (2), pp. 169-177. https://doi.org/10.1023/A:1020190918431
Early origins of the X and Y chromosomes: lessons from tilapia
Griffin, D., Harvey, S., Campos-Ramos, R., Ayling, L., Bromage, N., Masabanda, J. and Penman, D. 2002. Early origins of the X and Y chromosomes: lessons from tilapia. Cytogenetic and Genome Research. 99 (1-4), pp. 157-163. https://doi.org/10.1159/000071588
An investigation of sex determination in the Mozambique tilapia, Oreochromis mossambicus, using synaptonemal complex analysis, FISH, sex reversal and gynogenesis
Campos-Ramos, R., Harvey, S., McAndrew, B. and Penman, D. 2003. An investigation of sex determination in the Mozambique tilapia, Oreochromis mossambicus, using synaptonemal complex analysis, FISH, sex reversal and gynogenesis. Aquaculture. 221 (1-4), pp. 125-140. https://doi.org/10.1016/S0044-8486(03)00072-3
Physical mapping of the brain and ovarian aromatase genes in the Nile tilapia, Oreochromis niloticus, by fluorescence in situ hybridization
Harvey, S., Kwon, J. and Penman, D. 2003. Physical mapping of the brain and ovarian aromatase genes in the Nile tilapia, Oreochromis niloticus, by fluorescence in situ hybridization. Animal Genetics. 34 (1), pp. 62-64. https://doi.org/10.1046/j.1365-2052.2003.00941.x
Analysis of repetitive DNA sequences in the sex chromosomes of Oreochromis niloticus
Harvey, S., Boonphakdee, C., Campos-Ramos, R., Ezaz, M., Griffin, D., Bromage, N. and Penman, D. 2003. Analysis of repetitive DNA sequences in the sex chromosomes of Oreochromis niloticus. Cytogenetic and Genome Research. 101 (3-4), pp. 314-319. https://doi.org/10.1159/000074355
Isolation and physical mapping of sex-linked AFLP markers in Nile tilapia (Oreochromis niloticus L.)
Ezaz, M., Harvey, S., Boonphakdee, C., Teale, A., McAndrew, B. and Penman, D. 2004. Isolation and physical mapping of sex-linked AFLP markers in Nile tilapia (Oreochromis niloticus L.). Marine Biotechnology. 6 (5), pp. 435-445. https://doi.org/10.1007/s10126-004-3004-6
Thermal variation reveals natural variation between isolates of Caenorhabditis elegans
Harvey, S. and Viney, M. 2007. Thermal variation reveals natural variation between isolates of Caenorhabditis elegans. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution. 308B (4), pp. 409-416. https://doi.org/10.1002/jez.b.21161
Sex-specific differences in the synaptonemal complex in the genus Oreochromis (Cichlidae)
Campos-Ramos, R., Harvey, S. and Penman, D. 2009. Sex-specific differences in the synaptonemal complex in the genus Oreochromis (Cichlidae). Genetica. 135 (3), pp. 325-332. https://doi.org/10.1007/s10709-008-9280-8
Natural variation in gene expression in the early development of dauer larvae of Caenorhabditis elegans
Harvey, S., Barker, G., Shorto, A. and Viney, M. 2009. Natural variation in gene expression in the early development of dauer larvae of Caenorhabditis elegans. BMC Genomics. 10 (1), p. 325. https://doi.org/10.1186/1471-2164-10-325
Non-dauer larval dispersal in Caenorhabditis elegans
Harvey, S. 2009. Non-dauer larval dispersal in Caenorhabditis elegans. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution. 312B (3), pp. 224-230. https://doi.org/10.1002/jez.b.21287
The control of morph development in the parasitic nematode Strongyloides ratti
Harvey, S., Gemmill, A., Read, A. and Viney, M. 2000. The control of morph development in the parasitic nematode Strongyloides ratti. Proceedings of the Royal Society B: Biological Sciences. 267 (1457), pp. 2057-2063.