Energetic costs of mounting an immune response in a coral reef damselfish (<i>Pomacentrus amboinensis</i>)

Journal article

Levet, M., Roche, D., Killen, S., Colosio, Simona, Bshary, Redouan, Miest, Joanna J. and Binning, S. 2024. Energetic costs of mounting an immune response in a coral reef damselfish (<i>Pomacentrus amboinensis</i>). Canadian Journal of Zoology. 102 (4), pp. 380-392. https://doi.org/10.1139/cjz-2023-0033

Publication dates
Authors	Levet, M., Roche, D., Killen, S., Colosio, Simona, Bshary, Redouan, Miest, Joanna J. and Binning, S.
Abstract	While immune responses can be energetically costly, quantifying these costs is challenging. We tested the metabolic costs of immune activation in damselfish ( Pomacentrus amboinensis Bleeker, 1868) following a mass-adjusted injection of lipopolysaccharide (LPS) endotoxin. Fish were divided into eight treatments: two controls (handling and saline injection) and six LPS groups with concentrations ranging from 3 to 100 mg kg−1. We used intermittent flow respirometry to measure differences in oxygen uptake (delta ṀO2) 20 h before versus 20 h after LPS injection and changes in metabolic traits (lowest, routine, and peak metabolic rates) as proxies of the aerobic costs of metabolism. Spleen somatic index (SSI) and gene expression in spleens were measured to assess immune activation. We found no difference in metabolic traits or SSI but observed different nonlinear patterns of delta ṀO2 in fish exposed to 50 and 100 mg kg−1 LPS compared to lower doses and controls. Fish exposed to high doses of LPS also had lower residual aerobic scope compared to controls and lower LPS doses. Fish exposed to doses of 3, 50, and 100 mg kg−1 showed altered gene expression compared to the handling control. Overall, our results suggest that immune activation has measurable effects on metabolic traits that are both dose- and time-dependent.
Keywords	Animal Science and Zoology; Ecology, Evolution, Behavior and Systematics
Year	2024
Journal	Canadian Journal of Zoology
Journal citation	102 (4), pp. 380-392
Publisher	Canadian Science Publishing
ISSN	0008-4301
	1480-3283
Digital Object Identifier (DOI)	https://doi.org/10.1139/cjz-2023-0033
Official URL	https://cdnsciencepub.com/doi/abs/10.1139/cjz-2023-0033
Funder	Fonds de recherche du Québec – Nature et technologies
	Groupe de recherche interuniversitaire en limnologie
	Université de Neuchâtel
	Swiss Science Foundation
Online	15 Nov 2023
Print	01 Apr 2024
Publication process dates
Accepted	15 Nov 2023
Deposited	24 Jan 2024
Accepted author manuscript	Levet et al 2023.pdf License CC BY-NC-ND 4.0 File Access Level Open
Output status	Published
Additional information	Publications router: Date 2024-04-01 of type 'publication_date' with format 'print' included in notification Publications router: Date 2024-04-01 of type 'ppub' included in notification Publications router: Date 2024-04-01 of type 'issued' included in notification

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Accepted author manuscript

	Levet et al 2023.pdf
License: CC BY-NC-ND 4.0
File access level: Open

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