Characterisation of a virus from tomato

Masters Thesis

Smith, I. 2018. Characterisation of a virus from tomato. Masters Thesis Canterbury Christ Church University Faculty of Social and Applied Sciences
AuthorsSmith, I.
TypeMasters Thesis
Qualification nameMSc

Tomato (Solanum lycopersicum) material with mosaic symptoms was sent to the university by a local commercial glasshouse for examination. In order to determine the causative agent of any infection, a series of experiments was undertaken.

From initial analysis viral nucleic acid sequences were obtained by inoculating indicator plants with sap obtained from the diseased leaves, and it was shown that a nonmicrobial infectious agent was transmissible. Following this, it was found possible to extract viral nucleic acids from the inoculated plants, and the results suggested the possibility of a mixed infection by two pepino mosaic virus strains, as well as the presence of a possible Potyvirus. The rapid amplification of cDNA ends was the method used to attempt to obtain a full-length sequence of pepino mosaic virus. A partial sequence recovered corresponded to the genes encoding the Triple Gene Block and the coat protein of the viral genome. A rapid detection method using the dot blot technique was also explored and the results showed promise for future research in this area.

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Publication process dates
Deposited19 Mar 2019
Output statusUnpublished
Accepted author manuscript
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