Standard operating procedure for the operation of SEM-EDX for elemental analysis, with additional applications to biomass

Report


Hernandez, E., Thomas, J.K., Kheirandish, K., Wilson, C. and Ortega, H. 2024. Standard operating procedure for the operation of SEM-EDX for elemental analysis, with additional applications to biomass. Canterbury Christ Church University. https://doi.org/10.5281/zenodo.13736742
AuthorsHernandez, E., Thomas, J.K., Kheirandish, K., Wilson, C. and Ortega, H.
Abstract

This document and its content are an aider for our group, and it is not intended to be a replacement for the training, protocols, documents and else provided by the technical team.

This SOP is for the microscopic and elemental analysis of samples via SEM-EDX. It can be applied to all samples that are adequate for SEM-EDX. Additionally, the scope of this SOP covers the analyses of biomass and/or materials from living systems that are adequate for SEM-EDX.

KeywordsScanning electron microscopy; Elemental Analysis ; Energy dispersive X-ray analysis ; SEM EDX S; SEM-EDX Elemental Analysis ; SEM-EDX Protocol ; SEM-EDX Procedure ; SEM-EDX Method ; SEM-EDX Technique ; Biomass Analysis ; Elemental Composition
Year2024
PublisherCanterbury Christ Church University
Digital Object Identifier (DOI)https://doi.org/10.5281/zenodo.13736742
File
License
File Access Level
Open
Publication process dates
Deposited11 Sep 2024
FunderCanterbury Christ Church University (RKE ERC funded)
Publisher's version
File Access Level
Controlled
Additional information

How to cite:
Hernandez, J.E., Thomas, J.K. , Kheirandish, K., Wilson, C. and Ortega, H. (2024) Standard operating procedure for analysing of biomass through SEM-EDX. DOI: https://doi.org/10.5281/zenodo.13736742.

Permalink -

https://repository.canterbury.ac.uk/item/98yzz/standard-operating-procedure-for-the-operation-of-sem-edx-for-elemental-analysis-with-additional-applications-to-biomass

Download files


File
SOP_SEM-EDX_CCCU_1.1.pdf
License: CC BY 4.0
File access level: Open

  • 19
    total views
  • 9
    total downloads
  • 10
    views this month
  • 5
    downloads this month

Export as

Related outputs

Systems analysis for sustainable bioprocess engineering and biotechnology
Hernandez, J.E. 2024. Systems analysis for sustainable bioprocess engineering and biotechnology.
Sustainable agrobiorefinery system for advanced ethanol production from Opuntia prickly pear cactus nopales
Hernandez, J.E., Espinosa-Solares, T., Pérez-Cadena, R., Téllez-Jurado, A. and Ramírez-Arpide, F.R. 2024. Sustainable agrobiorefinery system for advanced ethanol production from Opuntia prickly pear cactus nopales. Energy Conversion and Management. 321, p. 119052. https://doi.org/10.1016/j.enconman.2024.119052
Differential microRNA expression in the SH-SY5Y human cell model as potential biomarkers for Huntington’s disease
Belkozhayev, Ayaz, Niyazova, Raigul, Kamal, Mohammad Amjad, Ivashchenko, Anatoliy, Sharipov, Kamalidin and Wilson, C. 2024. Differential microRNA expression in the SH-SY5Y human cell model as potential biomarkers for Huntington’s disease. Frontiers in Cellular Neuroscience. 18, p. 1399742. https://doi.org/10.3389/fncel.2024.1399742
Novel transcriptional and DNA methylation abnormalities of SORT1 Gene in non-small cell lung cancer
Acha-Sagredo, Amelia, Wilson, C., Garcia Bediaga, Naiara, Kalirai, Helen, Davies, M., Coupland, S., Field, John K and Liloglou, T. 2024. Novel transcriptional and DNA methylation abnormalities of SORT1 Gene in non-small cell lung cancer. Cancers. 16 (11), p. 2154. https://doi.org/10.3390/cancers16112154
Sustainable agriculture: Vertical farming, artificial intelligence and bio-fertiliser; the future of farming
Vaccaro, N. and Hernandez, E. 2024. Sustainable agriculture: Vertical farming, artificial intelligence and bio-fertiliser; the future of farming. https://doi.org/10.5281/zenodo.11485604
Editorial: Targeting nucleotide metabolism for enhancing antitumor immunity.
Wu, Jun, Rong, Yu, Li, Tian, Wilson, C., He, Yazhou, Chen, Danqian, Han, Jin and Zhang, Xingmei 2024. Editorial: Targeting nucleotide metabolism for enhancing antitumor immunity. Frontiers in Immunology. 15, p. 1412057. https://doi.org/10.3389/fimmu.2024.1412057
Nature inspired strategy to capture smoky carbon dioxide into commercial carbonates and add value to farming
Hernandez, E. 2024. Nature inspired strategy to capture smoky carbon dioxide into commercial carbonates and add value to farming.
Investigating the effect of mesoporous silica nanoparticles as a drug delivery system for Withaferin A in the treatment of Lung Cancer
Holder, J., Wilson, C., Bertolo-Pardo, E., Byrne, L., Lodeiro, C., Oliveira, E. and Trim, C. 2024. Investigating the effect of mesoporous silica nanoparticles as a drug delivery system for Withaferin A in the treatment of Lung Cancer.
Investigating the effect of silica nanoparticles as a drug delivery system for Doxorubicin in the treatment of lung cancer
Holder, J., Wilson, C., Bertolo-Pardo, E., Byrne, L., Oliveira, E., Lodeiro, C. and Trim, C. 2024. Investigating the effect of silica nanoparticles as a drug delivery system for Doxorubicin in the treatment of lung cancer.
AI is a viable alternative to high throughput screening: a 318-target study
Wilson, C.M., Byrne, L., Al-Yozbaki, M. and The Atomwise AIMS Program 2024. AI is a viable alternative to high throughput screening: a 318-target study. Scientific Reports. https://doi.org/10.1038/s41598-024-54655-z
Mesenchymal stem cell-derived exosomes: Characteristics and applications in disease pathology and management.
Padinharayil, H., Varghese, J., Wilson, C. and George, A. 2024. Mesenchymal stem cell-derived exosomes: Characteristics and applications in disease pathology and management. Life Sciences. 342 (122542). https://doi.org/10.1016/j.lfs.2024.122542
New challenger for sustainable ethanol production in industrial biorefineries
Hernandez, E., Espinosa-Solares, T., Cortés-Trejo, I., Téllez-Jurado and A. and Ramírez-Arpide, F.R. 2023. New challenger for sustainable ethanol production in industrial biorefineries.
Graphene-based nanomaterials and their application in bioreactors
Hernandez, J.E., Mohd Fuzi, S.F.Z. and Azman, N. A. 2023. Graphene-based nanomaterials and their application in bioreactors. in: Castro, G. R., Nguyen, T. A., Bilal, M., Nadda, A. K. and Sharma, S. (ed.) Nanomaterials for Bioreactors and Bioprocessing Applications Elsevier. pp. 19-42
Overview of hepatocellular carcinoma: from molecular aspects to future therapeutic options.
Panneerselvam, S., Wilson, Cornelia, Kumar, Prem, Abirami, Dinu, Pamarthi, Jayakrishna, Reddy, Mettu Srinivas and Varghese, Joy 2023. Overview of hepatocellular carcinoma: from molecular aspects to future therapeutic options. Cell Adhesion and Migration. 17 (1), pp. 1-21. https://doi.org/10.1080/19336918.2023.2258539
The use of nanoparticles for targeted drug delivery in non-small cell lung cancer.
Holder, Jessica E, Ferguson, Christopher, Oliveira, Elisabete, Lodeiro, Carlos, Trim, Carol M, Byrne, Lee J, Bertolo, Emilia and Wilson, Cornelia M 2023. The use of nanoparticles for targeted drug delivery in non-small cell lung cancer. Frontiers in Oncology. 13, p. 1154318. https://doi.org/10.3389/fonc.2023.1154318
Stochastic Variation in DNA Methylation Modulates Nucleosome Occupancy and Alternative Splicing in Arabidopsis thaliana
Jabre, Ibtissam, Chaudhary, S., Wilson, C., Staiger, D. and Syed, N. 2022. Stochastic Variation in DNA Methylation Modulates Nucleosome Occupancy and Alternative Splicing in Arabidopsis thaliana. Plants. 11 (9), p. 1105. https://doi.org/10.3390/plants11091105
The role of nanotechnology for diagnostic and therapy strategies in lung cancer
Holder, J., Wilson, C. and Al-Yozbaki, M. 2022. The role of nanotechnology for diagnostic and therapy strategies in lung cancer. in: Jeremić, B. (ed.) Advances in Radiation Oncology in Lung Cancer Cham Springer.
Caracterización fisicoquímica de la grasa de ovino y evaluación del efecto de la pre-esterificación y transesterificación en la producción de biodiesel
Hernandez, E., Pérez Cadena, R., González-Escamilla, E. and Delgadillo-López, A. E. 2022. Caracterización fisicoquímica de la grasa de ovino y evaluación del efecto de la pre-esterificación y transesterificación en la producción de biodiesel. Difu100ci@, Revista de difusión científica, ingeniería y tecnologías . 16 (3).
Non-small cell lung carcinoma (NSCLC): Implications on molecular pathology and advances in early diagnostics and therapeutics.
Padinharayil, Hafiza, Varghese, Jinsu, John, Mithun Chacko, Rajanikant, Golgodu Krishnamurthy, Wilson, Cornelia M, Al-Yozbaki, Minnatallah, Renu, Kaviyarasi, Dewanjee, Saikat, Sanyal, Rupa, Dey, Abhijit, Mukherjee, Anirban Goutam, Wanjari, Uddesh Ramesh, Gopalakrishnan, Abilash Valsala and George, Alex 2022. Non-small cell lung carcinoma (NSCLC): Implications on molecular pathology and advances in early diagnostics and therapeutics. Genes & Diseases. 10 (3), pp. 960-989. https://doi.org/10.1016/j.gendis.2022.07.023
The role of nanotechnology for diagnostic and therapy strategies in lung cancer
Holder, J., Al-Yozbaki, M. and Wilson, C. 2022. The role of nanotechnology for diagnostic and therapy strategies in lung cancer. in: Medical Radiology Berlin, Heidelberg Springer. pp. 1-18
Advances in the lung cancer immunotherapy approaches
Padinharayil, H., Alappat, R., Joy, Liji Maria, Anilkumar, K., Wilson, C., George, A., Valsala Gopalakrishnan, A., Madhyastha, H., Ramesh, Thiyagarajan, Sathiyamoorthi, Ezhaveni, Lee, Jintae and Ganesan, R. 2022. Advances in the lung cancer immunotherapy approaches. Vaccines. 10 (11), p. 1963. https://doi.org/10.3390/vaccines10111963
Interpretation of initial adhesion of Pseudomonas Putida on hematite and quartz using surface thermodynamics, DLVO, and XDLVO theories
Hernandez, E., Zuki, F.M., Pourzolfagh, H. and Edyvean, R.G.J. 2022. Interpretation of initial adhesion of Pseudomonas Putida on hematite and quartz using surface thermodynamics, DLVO, and XDLVO theories. Surface Engineering and Applied Electrochemistry. 58 (3).
Feedstocks and challenges to biofuel development
Hernandez, E., Botella, C., Diaz, A.B., Liang, Y. and Sivakumar, S.V. 2022. Feedstocks and challenges to biofuel development. in: Handbook of Biofuels Production Processes and Technologies Elsevier.
Proteomic analysis of the extracellular vesicles derived from human hip joints affected by osteoarthritis
Tirnoveanu, A., Ahmed, S., Webb, R., Howland, K., Hurt, A., Wilson, C. and Mylona, A. 2022. Proteomic analysis of the extracellular vesicles derived from human hip joints affected by osteoarthritis.
Bioinformatics analysis of the interaction of miRNAs and piRNAs with human mRNA genes having di- and Trinucleotide repeats
Belkozhayev, A., Niyazova, Raigul, Wilson, C., Jainakbayev, Nurlan, Pyrkova, A., Ashirbekov, Y., Akimniyazova, A., Sharipov, K. and Ivashchenko, A. 2022. Bioinformatics analysis of the interaction of miRNAs and piRNAs with human mRNA genes having di- and Trinucleotide repeats. Genes. 13 (5), p. 800. https://doi.org/10.3390/genes13050800
Real-time monitoring of healthy omega-3 production in micro-algae: A viability study
Hernandez, E. and Vaccaro, N.M. 2021. Real-time monitoring of healthy omega-3 production in micro-algae: A viability study.
Viability study on real-time monitoring of healthy omega-3 production in micro-algae
Hernandez, E. and Vaccaro, N.M. 2021. Viability study on real-time monitoring of healthy omega-3 production in micro-algae. UK BBSRC.
Extracellular vesicles, stem cells and the role of miRNAs in neurodegeneration
Belkozhayev, Ayaz M., Al-Yozbaki, Minnatallah, George, Alex, Ye Niyazova, Raigul, Sharipov, Kamalidin O., Byrne, Lee J. and Wilson, Cornelia M. 2021. Extracellular vesicles, stem cells and the role of miRNAs in neurodegeneration. Current Neuropharmacology. 20 (8), pp. 1450-1478. https://doi.org/10.2174/1570159x19666210817150141
Exploring the cytotoxic mechanisms of Pediocin PA-1 towards HeLa and HT29 cells by comparison to known bacteriocins: Microcin E492, enterocin heterodimer and Divercin V41.
Buss, G. and Wilson, C. 2021. Exploring the cytotoxic mechanisms of Pediocin PA-1 towards HeLa and HT29 cells by comparison to known bacteriocins: Microcin E492, enterocin heterodimer and Divercin V41. PLoS ONE. 16 (9), p. e0251951. https://doi.org/10.1371/journal.pone.0251951
The undiscovered potential of essential oils for treating SARS-CoV-2 (COVID-19)
Wilkin, P. J., Al-Yozbaki, M., George, A., Gupta, G.K. and Wilson, C.M. 2021. The undiscovered potential of essential oils for treating SARS-CoV-2 (COVID-19). Current Pharmaceutical Design. 26 (41), pp. 5261-5277. https://doi.org/10.2174/1381612826666201015154611
Balancing neurotrophin pathway and sortilin function: its role in human disease
Al-Yozbaki, M., Acha-Sagredo, A., George, A., Liloglou, T. and Wilson, C.M. 2021. Balancing neurotrophin pathway and sortilin function: its role in human disease. Biochim Biophys Acta Rev Cancer. 1874 (2). https://doi.org/10.1016/j.bbcan.2020.188429
Targeting DNA methyltransferases in non-small-cell lung cancer
Al-Yozbaki, M., Jabre, I., Syed, N. and Wilson, C. 2021. Targeting DNA methyltransferases in non-small-cell lung cancer. Seminars in Cancer Biology. https://doi.org/10.1016/j.semcancer.2021.01.005
Therapeutic potential of natural compounds in lung cancer
Al-Yozbaki, M., Wilkin, P., Gupta, G. and Wilson, C. 2021. Therapeutic potential of natural compounds in lung cancer. Current Medicinal Chemistry. 28 (39), pp. 7988-8002. https://doi.org/10.2174/0929867328666210322103906
Performance evaluation of a small scale digester for achieving decentralised
Gonzalez, R., Hernandez, E., Gomez, X., Smith, R., Gonzales Arias, J., Elias Martínez, J. and Blanco, D. 2020. Performance evaluation of a small scale digester for achieving decentralised. Waste Management. 118, pp. 99-109. https://doi.org/10.1016/j.wasman.2020.08.020
Differential nucleosome occupancy modulates alternative splicing in Arabidopsis thaliana
Jabre, I., Chaudhary, S., Guo, W., Kalyna, M., Reddy, A. S. N., Chen, W., Zhang, R., Wilson, C. and Syed, N. 2020. Differential nucleosome occupancy modulates alternative splicing in Arabidopsis thaliana. New Phytologist. https://doi.org/10.1111/nph.17062
Applications and strategies in nanodiagnosis and nanotherapy in lung cancer
Woodman, C., Vundu, G., George, A. and Wilson, C. 2020. Applications and strategies in nanodiagnosis and nanotherapy in lung cancer. Seminars in Cancer Biology. https://doi.org/10.1016/j.semcancer.2020.02.009
New approaches to engineering higher education: case studies
Nortcliffe, A., McIntosh, G. and Hernandez, E. 2019. New approaches to engineering higher education: case studies.
Dry weight model, capacitance and metabolic data as indicators of fungal biomass growth in solid state fermentation
Botella, Carolina, Hernandez, J.E. and Webb, Colin 2019. Dry weight model, capacitance and metabolic data as indicators of fungal biomass growth in solid state fermentation. Food and Bioproducts Processing. 114, pp. 144-153. https://doi.org/10.1016/j.fbp.2018.12.002
Toxicity and biodegradability of caffeic acid in anaerobic digesting sludge
Hernandez, E. and Edyvean, R.G.J. 2018. Toxicity and biodegradability of caffeic acid in anaerobic digesting sludge. Water SA. 44 (1). https://doi.org/10.4314/wsa.v44i1.04
Conceptualization, modelling and environmental impact assessment of a natural rubber techno-ecological system with nutrient, water and energy integration
Martinez-Hernandez, E. and Hernandez, E. 2018. Conceptualization, modelling and environmental impact assessment of a natural rubber techno-ecological system with nutrient, water and energy integration. Journal of Cleaner Production. 185, pp. 707-722. https://doi.org/10.1016/j.jclepro.2018.02.297
Entrepreneurial Intentions of university students in an emerging economy: The influence of university support and proactive personality on students' entrepreneurial intention
Hernandez, E., Mustafa, M.J,, Mahon, C. and Chee, L.K. 2016. Entrepreneurial Intentions of university students in an emerging economy: The influence of university support and proactive personality on students' entrepreneurial intention. Journal of Entrepreneurship in Emerging Economies. 8 (2), pp. 162-179. https://doi.org/10.1108/JEEE-10-2015-0058
New DNA sequences from bacteria converting phenol into acetate under strict anaerobic conditions
Hernandez, E. and Edyvean, R. 2016. New DNA sequences from bacteria converting phenol into acetate under strict anaerobic conditions. Journal of Medical and Bioengineering. 5 (1), pp. 1-10. https://doi.org/10.12720/jomb.5.1.1-10
The role of endoproteolytic processing in neurodegeneration
Wilson, C., Mushtaq, G., Kamal, M. and Terro, F. 2016. The role of endoproteolytic processing in neurodegeneration. CNS and Neurological Disorders Drug Targets. 15. https://doi.org/10.2174/1871527315666160922163511
Challenges and strategies in precision medicine for non-small cell lung cancer
Wilson, C., Al-Akhrass, A. and Sacco, J. 2016. Challenges and strategies in precision medicine for non-small cell lung cancer. Current Pharmaceutical Design. 22. https://doi.org/10.2174/1381612822666160603014932
Sortilin mediates the release and transfer of exosomes in concert with two tyrosine kinase receptors
Wilson, C., Naves, T., Vincent, F., Lalloue, F. and Jauberteau, M. 2015. Sortilin mediates the release and transfer of exosomes in concert with two tyrosine kinase receptors.
Sortilin mediates the release and transfer of exosomes in concert with two tyrosine kinase receptors.
Wilson, C., Naves, T., Vincent, F., Melloni, B., Bonnaud, F., Lalloue, F. and Jauberteau, M. 2014. Sortilin mediates the release and transfer of exosomes in concert with two tyrosine kinase receptors. Journal of Cell Science (JCS). 127 (18), pp. 3983-3997. https://doi.org/10.1242/jcs.149336
Development of prebiotics and prebiotics
Hernandez, E. and Pandiella, S. 2013. Development of prebiotics and prebiotics. in: Teixeira, J. A. and Vicente, A.A. (ed.) Engineering Aspects of Food Biotechnology Abingdon CRC Press. pp. 21-60
A novel process for enhancing oil production in algae biorefineries through bioconversion of solid by-products
Hernandez, E., Colin, W. and Trzcinski, A. 2012. A novel process for enhancing oil production in algae biorefineries through bioconversion of solid by-products. Bioresource Technology. 116, pp. 295-301. https://doi.org/10.1016/j.biortech.2012.03.078
Comparison between a two-stage and single-stage digesters when treating a synthetic wastewater contaminated with phenol
Hernandez, J. E. and Edyvean, R.G.J. 2011. Comparison between a two-stage and single-stage digesters when treating a synthetic wastewater contaminated with phenol. Water SA. 37 (1), pp. 1-6. https://doi.org/10.4314/wsa.v37i1.64103
Biogas production from cheese whey: Past, present and future
Hernandez, E., Bachmann, R.T. and Johnson, A.C 2008. Biogas production from cheese whey: Past, present and future. in: Cerdan, M.E., González-Siso, M.I and Becerra, M. (ed.) Advances in Cheese Whey Utilization Transworld Research Network. pp. 147-162
Inhibition of biogas production and biodegradability by substituted phenolic compounds in anaerobic sludge
Hernandez, E. and Edyvean, R.G.J. 2008. Inhibition of biogas production and biodegradability by substituted phenolic compounds in anaerobic sludge. Journal of Hazardous Materials. 160 (1), p. 20–28. https://doi.org/10.1016/j.jhazmat.2008.02.075
A cost-benefit analysis of methods for the determination of biomass concentration in wastewater treatment
Hernandez, J.E., Bachmann, R.T. and Edyvean, R.G.J. 2006. A cost-benefit analysis of methods for the determination of biomass concentration in wastewater treatment. Anaerobe. 12 (5-6), pp. 254-259. https://doi.org/10.1016/j.anaerobe.2006.09.005
Anaerobic treatment of phenol in a two-stage anaerobic reactor
Hernandez, J.E. and Edyvean, R.G.J. 2004. Anaerobic treatment of phenol in a two-stage anaerobic reactor. in: Brebbia, C.A., Kungolos, S., Popov, V. and Itoh, H. (ed.) Waste Management and the Environment II WIT Press. pp. 203-211