Energetic costs of mounting an immune response in a coral reef damselfish (Pomacentrus amboinensis)

Journal article

Levet, M., Roche, D., Killen, S., Colosio, Simona, Bshary, Redouan, Miest, Joanna J. and Binning, S. 2024. Energetic costs of mounting an immune response in a coral reef damselfish (Pomacentrus amboinensis). Canadian Journal of Zoology. 102 (4), pp. 380-392. https://doi.org/10.1139/cjz-2023-0033
AuthorsLevet, M., Roche, D., Killen, S., Colosio, Simona, Bshary, Redouan, Miest, Joanna J. and Binning, S.
AbstractWhile immune responses can be energetically costly, quantifying these costs is challenging. We tested the metabolic costs of immune activation in damselfish ( Pomacentrus amboinensis Bleeker, 1868) following a mass-adjusted injection of lipopolysaccharide (LPS) endotoxin. Fish were divided into eight treatments: two controls (handling and saline injection) and six LPS groups with concentrations ranging from 3 to 100 mg kg−1. We used intermittent flow respirometry to measure differences in oxygen uptake (delta ṀO2) 20 h before versus 20 h after LPS injection and changes in metabolic traits (lowest, routine, and peak metabolic rates) as proxies of the aerobic costs of metabolism. Spleen somatic index (SSI) and gene expression in spleens were measured to assess immune activation. We found no difference in metabolic traits or SSI but observed different nonlinear patterns of delta ṀO2 in fish exposed to 50 and 100 mg kg−1 LPS compared to lower doses and controls. Fish exposed to high doses of LPS also had lower residual aerobic scope compared to controls and lower LPS doses. Fish exposed to doses of 3, 50, and 100 mg kg−1 showed altered gene expression compared to the handling control. Overall, our results suggest that immune activation has measurable effects on metabolic traits that are both dose- and time-dependent.
KeywordsPomacentrus amboinensis; Metabolism; Gene expression; Spleen somatic index; Generalized additive mixed model; Damselfish
Year2024
JournalCanadian Journal of Zoology
Journal citation102 (4), pp. 380-392
PublisherCanadian Science Publishing
ISSN0008-4301
1480-3283
Digital Object Identifier (DOI)https://doi.org/10.1139/cjz-2023-0033
Official URLhttps://cdnsciencepub.com/doi/abs/10.1139/cjz-2023-0033
FunderFonds de recherche du Québec – Nature et technologies
Groupe de recherche interuniversitaire en limnologie
Université de Neuchâtel
Swiss Science Foundation
Publication dates
Online15 Nov 2023
Print01 Apr 2024
Publication process dates
Accepted15 Nov 2023
Deposited24 Jan 2024
Accepted author manuscript
License
File Access Level
Open
Output statusPublished
Additional information

Publications router: Date 2024-04-01 of type 'publication_date' with format 'print' included in notification
Publications router: Date 2024-04-01 of type 'ppub' included in notification
Publications router: Date 2024-04-01 of type 'issued' included in notification

Permalink -

https://repository.canterbury.ac.uk/item/96x0w/energetic-costs-of-mounting-an-immune-response-in-a-coral-reef-damselfish-pomacentrus-amboinensis

Download files

Accepted author manuscript
Levet et al 2023.pdf
License: CC BY-NC-ND 4.0
File access level: Open

  • 28
    total views
  • 14
    total downloads
  • 0
    views this month
  • 0
    downloads this month

Export as

Related outputs

Are fish immunocompetent enough to face climate change?
Franke, A., Beemelmanns, A. and Miest-Bray, J. 2024. Are fish immunocompetent enough to face climate change? Biology Letters. 20, p. 20230346. https://doi.org/10.1098/rsbl.2023.0346