Epigenetic differences in an identical genetic background modulate alternative splicing in A. thaliana.
Journal article
Chaudhary, Saurabh, Jabre, Ibtissam and Syed, Naeem 2021. Epigenetic differences in an identical genetic background modulate alternative splicing in A. thaliana. Genomics. 113 (6), pp. 3476-3486. https://doi.org/S0888-7543(21)00312-8
Authors | Chaudhary, Saurabh, Jabre, Ibtissam and Syed, Naeem |
---|---|
Abstract | How stable and temperature-dependent variations in DNA methylation and nucleosome occupancy influence alternative splicing (AS) remains poorly understood in plants. To answer this, we generated transcriptome, whole-genome bisulfite, and MNase sequencing data for an epigenetic Recombinant Inbred Line (epiRIL) of A. thaliana at normal and cold temperature. For comparative analysis, the same data sets for the parental ecotype Columbia (Col-0) were also generated, whereas for DNA methylation, previously published high confidence methylation profiles of Col-0 were used. Significant epigenetic differences in an identical genetic background were observed between Col-0 and epiRIL lines under normal and cold temperatures. Our transcriptome data revealed that differential DNA methylation and nucleosome occupancy modulate expression levels of many genes and AS in response to cold. Collectively, DNA methylation and nucleosome levels exhibit characteristic patterns around intron-exon boundaries at normal and cold conditions, and any perturbation in them, in an identical genetic background is sufficient to modulate AS in Arabidopsis. [Abstract copyright: Crown Copyright © 2021. Published by Elsevier Inc. All rights reserved.] |
Keywords | epiRILs; Nucleosome occupancy; Alternative splicing; DNA-methylation; Cold stress; Arabidopsis |
Year | 2021 |
Journal | Genomics |
Journal citation | 113 (6), pp. 3476-3486 |
Publisher | Elsevier |
ISSN | 1089-8646 |
Digital Object Identifier (DOI) | https://doi.org/S0888-7543(21)00312-8 |
https://doi.org/10.1016/j.ygeno.2021.08.006 | |
Official URL | https://www.sciencedirect.com/science/article/pii/S0888754321003128 |
Publication dates | |
Online | 12 Aug 2021 |
Publication process dates | |
Accepted | 10 Aug 2021 |
Deposited | 04 Jul 2024 |
Publisher's version | File Access Level Open |
Output status | Published |
Additional information | 0888-7543/Crown Copyright © 2021 Published by Elsevier Inc. This article is made available under the Elsevier license (http://www.elsevier.com/openaccess/userlicense/1.0/). |
Permalink -
https://repository.canterbury.ac.uk/item/94801/epigenetic-differences-in-an-identical-genetic-background-modulate-alternative-splicing-in-a-thaliana
Download files
7
total views2
total downloads4
views this month1
downloads this month