Supplementation of porcin in vitro maturation medium with FGF2, LIF, and IGF1 enhances cytoplasmic maturation in prepubertal gilts oocytes and improves embryo quality

Journal article

Serrano Albal, M., Silvestri, G., Kiazim, Lucas G., Vining, Lucy M., Zak, Louisa J., Walling, Grant A., Haigh, Alexandra M., Harvey, Simon C., Harvey, Katie E. and Griffin, Darren K. 2022. Supplementation of porcin in vitro maturation medium with FGF2, LIF, and IGF1 enhances cytoplasmic maturation in prepubertal gilts oocytes and improves embryo quality. Zygote (Cambridge, England). https://doi.org/10.1017/S0967199422000284
AuthorsSerrano Albal, M., Silvestri, G., Kiazim, Lucas G., Vining, Lucy M., Zak, Louisa J., Walling, Grant A., Haigh, Alexandra M., Harvey, Simon C., Harvey, Katie E. and Griffin, Darren K.
AbstractIn porcine production (IVP) systems, the use of oocytes derived from prepubertal gilts, whilst being commercially attractive, remains challenging due to their poor developmental competence following maturation (IVM). Follicular fluid contains important growth factors and plays a key role during oocyte maturation; therefore, it is a common supplementation for porcine IVM medium. However, follicular fluid contains many poorly characterized components, is batch variable, and its use raises biosecurity concerns. In an effort to design a defined IVM system, growth factors such as cytokines have been previously tested. These include leukaemia inhibitory factor (LIF), fibroblast growth factor 2 (FGF2), and insulin-like growth factor 1 (IGF1), the combination of which is termed 'FLI'. Here, using abattoir-derived oocytes in a well established porcine IVP system, we compared follicular fluid and FLI supplementation during both IVM and embryo culture to test the hypothesis that FLI can substitute for follicular fluid without compromising oocyte nuclear and cytoplasmic maturation. We demonstrate that in oocytes derived from prepubertal gilts, FLI supplementation enhances oocyte meiotic maturation and has a positive effect on the quality and developmental competence of embryos. Moreover, for the first time, we studied the effects of follicular fluid and FLI combined showing no synergistic effects.
KeywordsIn vitro fertilization; Pig; Blastocyst; COCs
Year2022
JournalZygote (Cambridge, England)
PublisherCambridge University Press
ISSN1469-8730
Digital Object Identifier (DOI)https://doi.org/10.1017/S0967199422000284
Official URLhttps://doi.org/10.1017/S0967199422000284
Publication dates
Online01 Sep 2022
Publication process dates
Deposited29 Sep 2022
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