Bioinformatics analysis of the interaction of miRNAs and piRNAs with human mRNA genes having di- and Trinucleotide repeats
Journal article
Belkozhayev, A., Niyazova, Raigul, Wilson, C., Jainakbayev, Nurlan, Pyrkova, A., Ashirbekov, Y., Akimniyazova, A., Sharipov, K. and Ivashchenko, A. 2022. Bioinformatics analysis of the interaction of miRNAs and piRNAs with human mRNA genes having di- and Trinucleotide repeats. Genes. 13 (5), p. 800. https://doi.org/10.3390/genes13050800
| Authors | Belkozhayev, A., Niyazova, Raigul, Wilson, C., Jainakbayev, Nurlan, Pyrkova, A., Ashirbekov, Y., Akimniyazova, A., Sharipov, K. and Ivashchenko, A. |
|---|---|
| Abstract | The variability of nucleotide repeats is considered one of the causes of diseases, but their biological function is not understood. In recent years, the interaction of miRNAs and piRNAs with the mRNAs of genes responsible for developing neurodegenerative and oncological diseases and diabetes have been actively studied. We explored candidate genes with nucleotide repeats to predict associations with miRNAs and piRNAs. The parameters of miRNAs and piRNA binding sites with mRNAs of human genes having nucleotide repeats were determined using the MirTarget program. This program defines the start of the initiation of miRNA and piRNA binding to mRNAs, the localization of miRNA and piRNA binding sites in the 5′-untranslated region (5′UTR), coding sequence (CDS) and 3′-untranslated region (3′UTR); the free energy of binding; and the schemes of nucleotide interactions of miRNAs and piRNAs with mRNAs. The characteristics of miRNAs and piRNA binding sites with mRNAs of 73 human genes were determined. The 5′UTR, 3′UTR and CDS of the mRNAs of genes are involved in the development of neurodegenerative, oncological and diabetes diseases with GU, AC dinucleotide and CCG, CAG, GCC, CGG, CGC trinucleotide repeats. The associations of miRNAs, piRNAs and candidate target genes could be recommended for developing methods for diagnosing diseases, including neurodegenerative diseases, oncological diseases and diabetes. |
| Keywords | mRNA; Gene; Diabetes; Neurodegenerative Disorders; Pirna; Mirna Binding; Oncological Diseases; Nucleotide Repeats; Humans; Nucleotides; MicroRNAs; RNA, Small Interfering; RNA, Messenger; 3' Untranslated Regions; 5' Untranslated Regions; Computational Biology; Trinucleotide Repeats |
| Year | 2022 |
| Journal | Genes |
| Journal citation | 13 (5), p. 800 |
| Publisher | MDPI AG |
| ISSN | 2073-4425 |
| Digital Object Identifier (DOI) | https://doi.org/10.3390/genes13050800 |
| Official URL | https://www.mdpi.com/2073-4425/13/5/800 |
| Funder | Science Committee of the Ministry of Education and Science of the Republic of Kazakhstan |
| Publication dates | |
| 01 Apr 2022 | |
| Online | 29 Apr 2022 |
| Publication process dates | |
| Accepted | 27 Apr 2022 |
| Deposited | 30 Nov 2022 |
| Publisher's version | |
| Output status | Published |
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https://repository.canterbury.ac.uk/item/91061/bioinformatics-analysis-of-the-interaction-of-mirnas-and-pirnas-with-human-mrna-genes-having-di-and-trinucleotide-repeats
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