Characterization of wild-type and STAT3 signaling-suppressed mesenchymal stem cells obtained from hemovac blood concentrates.

Journal article

Lee, Dong Hwan, Kim, Seon Ae, Go, Eun Jeong, Yoon, Chi Young, Cho, Mi-La, Shetty, Asode Ananthram and Kim, Seok Jung 2021. Characterization of wild-type and STAT3 signaling-suppressed mesenchymal stem cells obtained from hemovac blood concentrates. Annals of Translational Medicine. 9 (16), p. 1284. https://doi.org/10.21037/atm-21-791
AuthorsLee, Dong Hwan, Kim, Seon Ae, Go, Eun Jeong, Yoon, Chi Young, Cho, Mi-La, Shetty, Asode Ananthram and Kim, Seok Jung
AbstractVenous blood drained from the knee joint after total knee arthroplasty (TKA) using a hemovac line is a potential source of bone marrow components, including stem cells, from the cutting surface of cancellous bones of the knee joint. However, the function of mesenchymal stem cells (MSCs) in patients with osteoarthritis (OA-MSCs) can be disrupted by inflammation of the joint. Further, to override the invasive nature of the currently used methods to obtain stem cells, their functional modification is necessary for therapeutic applications. The effects of signal transducer and activator of transcription 3 (STAT3) signaling suppression on MSCs (iSTAT3-MSCs) were evaluated by comparative analyses of the characteristics of OA-MSCs and iSTAT3-MSCs from 20 patients who underwent TKA. OA-MSCs and iSTAT3-MSCs were adherent, with fibroblast-like appearance and high rates of expression of MSC-specific markers, including CD73, CD90, and CD105 (>90%). Both OA-MSCs and iSTAT3-MSCs were able to differentiate into osteogenic, adipogenic, and chondrogenic cells; however, iSTAT3-MSCs showed higher levels of osteogenic and chondrogenic differentiation markers than OA-MSCs. Additionally, the anti-inflammatory and chondroprotective cytokine levels were higher in iSTAT3-MSCs than in OA-MSCs. These findings indicate that iSTAT3-MSCs after TKA are potentially effective for stem cell therapy in the context of bone and cartilage disorders. [Abstract copyright: 2021 Annals of Translational Medicine. All rights reserved.]
KeywordsTotal knee arthroplasty (TKA); Signal transducer and activator of transcription 3 (STAT3); Osteoarthritis (OA); Stem cell therapy; Hemovac
Year2021
JournalAnnals of Translational Medicine
Journal citation9 (16), p. 1284
PublisherAME Publishing
ISSN2305-5839
Digital Object Identifier (DOI)https://doi.org/10.21037/atm-21-791
Related URLhttps://atm.amegroups.com/article/view/76931/html
Publication dates
OnlineAug 2021
Publication process dates
Accepted28 Jun 2021
Deposited01 Nov 2021
Output statusPublished
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