Study and preparation of Multifunctional Poly(L-Lysine)@Hyaluronic Acid Nanopolyplexes for the effective delivery of tumor suppressive MiR-34a into triple-negative breast cancer cells

Journal article


Djafari, J, Fernández-Lodeiro, J., Santos, H.M., Lorenzo, J., Rodriguez-Calado, S., Bertolo-Pardo, E., Capelo-Martínez, J.L. and Lodeiro, C 2020. Study and preparation of Multifunctional Poly(L-Lysine)@Hyaluronic Acid Nanopolyplexes for the effective delivery of tumor suppressive MiR-34a into triple-negative breast cancer cells. Materials. 13 (23), p. 5309. https://doi.org/10.3390/ma13235309
AuthorsDjafari, J, Fernández-Lodeiro, J., Santos, H.M., Lorenzo, J., Rodriguez-Calado, S., Bertolo-Pardo, E., Capelo-Martínez, J.L. and Lodeiro, C
Abstract

Non-viral gene delivery using exogenous microRNAs is a potential strategy for fighting cancers with poor prognosis and which lack specific therapies, such as triple-negative breast cancer (TNBC). Herein we report the synthesis of six nontoxic electrostatic polymeric nanocapsules (P1 to P6) for microRNA delivery in TNBC cells. 1H Nuclear Magnetic Resonance (NMR) spectroscopy and Scanning Electron Microscopy (SEM) were used to characterize the nanopolyplexes, synthesized with Poly(L-Lysine) and hyaluronic acid (Ha). Studies on the activity of the ternary HA/PLI/miRNA-34 nanopolyplexes towards TNBC cell line MDA-MB-231 were conducted. The nanopolyplexes mediated intracellular restoration of tumor suppressor miR34a was evaluated by using Western blotting to quantify the expression level of the Bcl-2 protein. The results suggest that the P5, with a ratio PLI/Ha of 0.05, was the most promising for the delivery of miR-34a into TNBC cells; the P5 nanocapsules were able to reduce Bcl-2 expression at a protein level, and had an effect in the overall cell viability after 24 h treatment.

KeywordsPolyplexes; RNA; Poly-Lysine; Triple negative breast cancer
Year2020
JournalMaterials
Journal citation13 (23), p. 5309
PublisherMDPI
ISSN1996-1944
Digital Object Identifier (DOI)https://doi.org/10.3390/ma13235309
Official URLhttps://doi.org/10.3390/ma13235309
Publication dates
Online24 Nov 2020
Publication process dates
Accepted18 Nov 2020
Deposited26 Nov 2020
Output statusPublished
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https://repository.canterbury.ac.uk/item/8wvz1/study-and-preparation-of-multifunctional-poly-l-lysine-hyaluronic-acid-nanopolyplexes-for-the-effective-delivery-of-tumor-suppressive-mir-34a-into-triple-negative-breast-cancer-cells

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