Developing methods of measuring and manipulating melanocyte/keratinocyte ratios to inform potential treatment of vitiligo vulgaris

Introduction
My doctoral work focuses on the disease vitiligo vulgaris. Several treatments already exist however, one totally successful remedy is unavailable. I have focused on cell-based technologies to refine the melanocyte–keratinocyte transplantation procedure (MKTP) that is currently in use. Treating this disease is important not just for its physical but its emotional effects.

Aim and objectives
The broad aim of my doctoral work was to establish techniques to enhance the MKTP vitiligo treatment by developing a method for isolating melanocyte and keratinocyte cells from skin, co-culturing in different ratios and manipulating this by different factors.

Methods
A co-culture system of melanocytes and keratinocytes at different ratios (1:3, 1:5 and 1:10) was established. Co-culture at different ratios was exposed to various concentrations of αMSH (2.5 µg/ml, 5 µg/ml and 10 µg/ml) and UVB (10 mJ/cm2, 20 mJ/cm2 and 30 mJ/cm2). Cells were evaluated for changes in morphology, melanin content, tyrosinase activity and the expression of selected markers (S100, TYRP1, TYR, pan-cytokeratin, cytokeratin 10 and filaggrin).