Enhanced bone marrow derived mesenchymal stem cell differentiation when isolated and expanded with human platelet rich plasma and differentiation media is supplemented with vitamin D
Journal article
Thapa, G., Tirnoveanu, A., Mylona, A. and Webb, R. 2020. Enhanced bone marrow derived mesenchymal stem cell differentiation when isolated and expanded with human platelet rich plasma and differentiation media is supplemented with vitamin D. eCM Periodicals & Conferences.
Authors | Thapa, G., Tirnoveanu, A., Mylona, A. and Webb, R. |
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Abstract | Vitamin D3 is well known to be involved in bone formation during foetal development and has been shown to be actively involved from gestational day 13 during foetal rat development [1]. Vitamin D3 and its associated enzyme 1-alpha-hydroxylase a member of the cytochrome P450 super family and encoded for by the gene CYP27B1. In this study we supplemented osteogenic media with vitamin D at a concentration of 10-4M and differentiated for 21 days with samples analysed by means of quantitative alizarin red assay and qPCR for the bone markers RUNX2, ALPL and HPRT1 |
Keywords | Cell therapy; Developmental biology |
Year | 2020 |
Journal | eCM Periodicals & Conferences |
Publisher | ECM Journal.com |
ISSN | 2522-235X |
Official URL | https://www.ecmconferences.org/abstracts/2020/Collection1/collection%201_oral.pdf |
Related URL | https://www.ecmconferences.org/abstracts/2020/Collection1/c1.html |
Funder | Canterbury Christ Church University |
Publication dates | |
20 Oct 2020 | |
Publication process dates | |
Deposited | 21 Oct 2020 |
Accepted | 20 Sep 2020 |
Accepted author manuscript | |
Output status | Published |
References | Bikle, D.D. Vitamin D and Bone. Curr Osteoporos Rep, 2012. 10(2): p151-9 |
https://repository.canterbury.ac.uk/item/8w889/enhanced-bone-marrow-derived-mesenchymal-stem-cell-differentiation-when-isolated-and-expanded-with-human-platelet-rich-plasma-and-differentiation-media-is-supplemented-with-vitamin-d
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