Enhanced bone marrow derived mesenchymal stem cell differentiation when isolated and expanded with human platelet rich plasma and differentiation media is supplemented with vitamin D

Journal article


Thapa, G., Tirnoveanu, A., Mylona, A. and Webb, R. 2020. Enhanced bone marrow derived mesenchymal stem cell differentiation when isolated and expanded with human platelet rich plasma and differentiation media is supplemented with vitamin D. eCM Periodicals & Conferences.
AuthorsThapa, G., Tirnoveanu, A., Mylona, A. and Webb, R.
Abstract

Vitamin D3 is well known to be involved in bone formation during foetal development and has been shown to be actively involved from gestational day 13 during foetal rat development [1]. Vitamin D3 and its associated enzyme 1-alpha-hydroxylase a member of the cytochrome P450 super family and encoded for by the gene CYP27B1. In this study we supplemented osteogenic media with vitamin D at a concentration of 10-4M and differentiated for 21 days with samples analysed by means of quantitative alizarin red assay and qPCR for the bone markers RUNX2, ALPL and HPRT1

KeywordsCell therapy; Developmental biology
Year2020
JournaleCM Periodicals & Conferences
PublisherECM Journal.com
ISSN2522-235X
Official URLhttps://www.ecmconferences.org/abstracts/2020/Collection1/collection%201_oral.pdf
Related URLhttps://www.ecmconferences.org/abstracts/2020/Collection1/c1.html
FunderCanterbury Chirst Church University
Publication dates
Print20 Oct 2020
Publication process dates
Deposited21 Oct 2020
Accepted20 Sep 2020
Accepted author manuscript
Output statusPublished
References

Bikle, D.D. Vitamin D and Bone. Curr Osteoporos Rep, 2012. 10(2): p151-9

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https://repository.canterbury.ac.uk/item/8w889/enhanced-bone-marrow-derived-mesenchymal-stem-cell-differentiation-when-isolated-and-expanded-with-human-platelet-rich-plasma-and-differentiation-media-is-supplemented-with-vitamin-d

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