Enhanced bone marrow derived mesenchymal stem cell differentiation when isolated and expanded with human platelet rich plasma and differentiation media is supplemented with vitamin D

Journal article


Thapa, G., Tirnoveanu, A., Mylona, A. and Webb, R. 2020. Enhanced bone marrow derived mesenchymal stem cell differentiation when isolated and expanded with human platelet rich plasma and differentiation media is supplemented with vitamin D. eCM Periodicals & Conferences.
AuthorsThapa, G., Tirnoveanu, A., Mylona, A. and Webb, R.
Abstract

Vitamin D3 is well known to be involved in bone formation during foetal development and has been shown to be actively involved from gestational day 13 during foetal rat development [1]. Vitamin D3 and its associated enzyme 1-alpha-hydroxylase a member of the cytochrome P450 super family and encoded for by the gene CYP27B1. In this study we supplemented osteogenic media with vitamin D at a concentration of 10-4M and differentiated for 21 days with samples analysed by means of quantitative alizarin red assay and qPCR for the bone markers RUNX2, ALPL and HPRT1

KeywordsCell therapy; Developmental biology
Year2020
JournaleCM Periodicals & Conferences
PublisherECM Journal.com
ISSN2522-235X
Official URLhttps://www.ecmconferences.org/abstracts/2020/Collection1/collection%201_oral.pdf
Related URLhttps://www.ecmconferences.org/abstracts/2020/Collection1/c1.html
FunderCanterbury Christ Church University
Publication dates
Print20 Oct 2020
Publication process dates
Deposited21 Oct 2020
Accepted20 Sep 2020
Accepted author manuscript
Output statusPublished
References

Bikle, D.D. Vitamin D and Bone. Curr Osteoporos Rep, 2012. 10(2): p151-9

Permalink -

https://repository.canterbury.ac.uk/item/8w889/enhanced-bone-marrow-derived-mesenchymal-stem-cell-differentiation-when-isolated-and-expanded-with-human-platelet-rich-plasma-and-differentiation-media-is-supplemented-with-vitamin-d

Download files


Accepted author manuscript
  • 136
    total views
  • 28
    total downloads
  • 1
    views this month
  • 0
    downloads this month

Export as

Related outputs

A-294 duplicate testing of samples suspected of SARS-CoV-2 infection could prevent misdiagnosis of Covid-19
Sánchez-Sánchez, J, Thapa, G, Mylona, A and Vuono, R 2023. A-294 duplicate testing of samples suspected of SARS-CoV-2 infection could prevent misdiagnosis of Covid-19. Clinical Chemistry. 69 (Supplement_1). https://doi.org/10.1093/clinchem/hvad097.259
Study on osteoarthritic joint: regenerative potential and disease markers
Tirnoveanu, A. 2022. Study on osteoarthritic joint: regenerative potential and disease markers. PhD Thesis Canterbury Christ Church University School of Psychology and Life Sciences
Bench to Bedside (research based evidence in Aesthetics)
Webb, R. 2022. Bench to Bedside (research based evidence in Aesthetics).
The development and validation of a low-cost trans perineal (TP) prostate biopsy simulator from 3D printed mould: improving trainees’ confidence and cognitive targeting skills
Ong, K.O., Thapa, G., Webb, R., Rahman, E., Dryhurst, D.D., Lunawat, R.L. and Sriprasad, S. 2022. The development and validation of a low-cost trans perineal (TP) prostate biopsy simulator from 3D printed mould: improving trainees’ confidence and cognitive targeting skills. World Journal of Urology.
Utilising DOE to optimise hydrogel composition for tissue engineering
UPTON, A., Tirnoveanu, A., Thapa, G., Zimbitas, G., Mylona, A. and Webb, R. 2022. Utilising DOE to optimise hydrogel composition for tissue engineering.
Diagnostic outcomes between home sleep apnoea testing and in-laboratory polysomnography: systematic review
Rotimi, O., Webb, R. and Rahman, E. 2022. Diagnostic outcomes between home sleep apnoea testing and in-laboratory polysomnography: systematic review.
Single vs replicate Real-Time PCR SARS-CoV-2 testing: Lessons learned for effective pandemic management.
Webb, William R., Thapa, Gauri, Tirnoveanu, Alice, Kallu, Sabrina, Loo Jin Yi, Charlene, Shah, Nirali, Macari, Joseph, Mitchell, Sadie, Fagg, Graham J, Jeremiah, Rachael N, Theminimulle, Sandiya, Vuono, Romina and Mylona, A. 2022. Single vs replicate Real-Time PCR SARS-CoV-2 testing: Lessons learned for effective pandemic management. PLoS ONE. 17 (7), p. e0269883. https://doi.org/10.1371/journal.pone.0269883
Rheological properties of bioinks for printing optimisation
Upton, A., Thapa, G., Zimbitas, G., Mylona, A. and Webb, R. 2022. Rheological properties of bioinks for printing optimisation.
Proteomic analysis of the extracellular vesicles derived from human hip joints affected by osteoarthritis
Tirnoveanu, A., Ahmed, S., Webb, R., Howland, K., Hurt, A., Wilson, C. and Mylona, A. 2022. Proteomic analysis of the extracellular vesicles derived from human hip joints affected by osteoarthritis.
Auricular reconstruction: where are we now? A critical literature review
Humphries, S., Joshi, A., Webb, R. and Kanegaonkar, R. 2021. Auricular reconstruction: where are we now? A critical literature review. European Archives of Oto-Rhino-Laryngology. https://doi.org/10.1007/s00405-021-06903-5
Update on the role of emerging stem cell technology in head and neck medicine
Spencer, H., Moshkbouymatin, N. A., Webb, W.R., Joshi, A. and D'Souza, A. 2021. Update on the role of emerging stem cell technology in head and neck medicine. Head and Neck. 43 (6), pp. 1928-1938. https://doi.org/10.1002/hed.26674
Poly (3-hydroxybutyrate-co-3-hydroxyhexanoate)/collagen hybrid scaffolds for tissue engineering applications.
Lomas, A., Webb, R., Han, J., Chen, G., Sun, X., Zhang, Z., El Haj, A. and Forsyth, N. 2013. Poly (3-hydroxybutyrate-co-3-hydroxyhexanoate)/collagen hybrid scaffolds for tissue engineering applications. Tissue Engineering. 19 (8). https://doi.org/10.1089/ten.TEC.2012.0457
The application of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) scaffolds for tendon repair in the rat model
Webb, R., Dale, T., Lomas, A., Zeng, G., Wimpenny, I., El Haj, A., Forsyth, N. and Chen, G. 2013. The application of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) scaffolds for tendon repair in the rat model. Biomaterials. 34 (28), pp. 6683-6694. https://doi.org/10.1016/j.biomaterials.2013.05.041
Sustained PDGF-BB release from PHBHHx loaded nanoparticles in 3D hydrogel/stem cell model
Dong, C., Webb, R., Peng, Q., Tang, J., Forsyth, N., Chen, G. and Haj, A. 2014. Sustained PDGF-BB release from PHBHHx loaded nanoparticles in 3D hydrogel/stem cell model. Journal of Biomedical Materials Research Part A. 103 (1), pp. 282-288.
Controlled production of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) nanoparticles for targeted and sustained drug delivery
Heathman, T., Webb, R., Han, J., Dan, Z., Chen, G., Forsyth, N., El Haj, A., Zhang, Z. and Sun, X. 2014. Controlled production of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) nanoparticles for targeted and sustained drug delivery. European Journal of Pharmaceutical Sciences. 103 (8), pp. 2498-2508. https://doi.org/10.1002/jps.24035
Opportunities for zonal cartilage regeneration: progenitor cell-laden hydrogels and bioprinting
Levato, R., Mouser, V., Webb, R., Otto, I., Gawlitta, D., van Weeren, P., Khan, I. and Malda, J. 2016. Opportunities for zonal cartilage regeneration: progenitor cell-laden hydrogels and bioprinting.
Tenogenic differentiation of human embryonic stem cells
Dale, T., Mazher, S., Webb, R., Zhou, J., Maffulli, N., Chen, G., El Haj, A. and Forsyth, N. 2018. Tenogenic differentiation of human embryonic stem cells. Tissue Engineering. 5-6, pp. 361-368. https://doi.org/10.1089/ten.tea.2017.0017
Macro-porous micro-carriers as a vehicle for chondroprogenitor expansion and scaffold formation for the rapid biofabrication of osteochondral implants
Webb, R., Levato, R., Moshkbouymatin, N., Zhang, Y., Francis, L., van Weeren, P., Malda, J. and Khan, I. 2017. Macro-porous micro-carriers as a vehicle for chondroprogenitor expansion and scaffold formation for the rapid biofabrication of osteochondral implants.
The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells
Levato, R., Webb, R., Otto, I., Mensinga, A., Zhang, Y., van Rijen, M., van Weeren, P., Khan, I. and Malda, J. 2017. The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells. Acta Biomaterialia. 61, pp. 41-53. https://doi.org/10.1016/j.actbio.2017.08.005
Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture
Otto, I., Levato, R., Webb, R., Khan, I., Breugem, C. and Malda, J. 2018. Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture. European Cells & Materials. 35, pp. 132-150. https://doi.org/10.22203/eCM.v035a10
Isolation of mesenchymal stem cells from bone marrow aspirate
Markides, H., Webb, W.R., El Haj, A.J., Chippendale, T., Coopman, K., Rafiq, Q. and Hewitt, C. 2019. Isolation of mesenchymal stem cells from bone marrow aspirate. in: Moo-Young, M. (ed.) Comprehensive Biotechnology Elsevier. pp. 137-148
Progenitor cells in auricular cartilage demonstrate promising cartilage regenerative potential in 3D hydrogel culture
Otto, I. A, Levatto, R., Webb, R., Khan I. M., Breugem, C.C. and Malda, J. 2017. Progenitor cells in auricular cartilage demonstrate promising cartilage regenerative potential in 3D hydrogel culture.
Mesenchymal inflammation drives genotoxic stress in hematopoietic stem cells and predicts disease evolution in human pre-leukemia
Zambetti, N., Ping, Z., Chen, S., Kenswill, K., Mylona, A., Sanders, M., Hoogenboezem, R., Bindels, E., Adisty, N., Van Strien, P., van der Leije, C., Westers, T., Cremers, E., Milanese, C., Mastroberardino, P., van Leeuwen, J., van der Eerden, B., Touw, I., Kuijpers, T., Kanaar, R., van der Loosdrecht, A., Vogl, T. and Raaijmakers, M. 2016. Mesenchymal inflammation drives genotoxic stress in hematopoietic stem cells and predicts disease evolution in human pre-leukemia. Cell Stem Cell. 19 (5), pp. 613-627. https://doi.org/10.1016/j.stem.2016.08.021
The Isl1/Ldb1 complex orchestrates heart-specific chromatin organization and transcriptional regulation
Caputo, L., Witzel, H., Kolovos, P., Cheedipudi, S., Looso, M., Mylona, A., van Ijcken, W., Evans, S., Braun, T., Soler, E., Grosveld, F. and Dobreva, G. 2015. The Isl1/Ldb1 complex orchestrates heart-specific chromatin organization and transcriptional regulation. Cell Stem Cell. 17 (3), pp. 287-299. https://doi.org/10.1016/j.stem.2015.08.007
Massive parallel RNA sequencing of highly purified mesenchymal elements in low-risk MDS reveals tissue-context dependent activation of inflammatory programs
Chen, S., Zambetti, N., Bindels, E., Kenswill, K., Mylona, A., Adisty, N., Hoogenboezem, R., Sanders, M., Cremers, E., Jansen, J., van de Loosdrecht, A. and Raaijmakers, M. 2016. Massive parallel RNA sequencing of highly purified mesenchymal elements in low-risk MDS reveals tissue-context dependent activation of inflammatory programs. Leukemia. https://doi.org/10.1038/leu.2016.91
Genome-wide analysis shows that Ldb1 controls essential hematopoietic genes/pathways in mouse early development and reveals novel players in hematopoiesis.
Mylona, A., Andrieu-Soler, C., Thongjuea, S., Martella, A., Soler, E., Jorna, R., Hou, J., Kockx, C., van Ijcken, W., Lenhard, B. and Grosveld, F. 2013. Genome-wide analysis shows that Ldb1 controls essential hematopoietic genes/pathways in mouse early development and reveals novel players in hematopoiesis. Blood. 121 (15). https://doi.org/10.1182/blood-2012-11-467654
Genomic structure and cloning of two transcript isoforms of human Sp8.
Mylona, A., Gough, J. and Edgar, A. 2004. Genomic structure and cloning of two transcript isoforms of human Sp8. BMC Genomics. 5 (86). https://doi.org/10.1186/1471-2164-5-86