Sustained PDGF-BB release from PHBHHx loaded nanoparticles in 3D hydrogel/stem cell model

Journal article

Dong, C., Webb, R., Peng, Q., Tang, J., Forsyth, N., Chen, G. and Haj, A. 2014. Sustained PDGF-BB release from PHBHHx loaded nanoparticles in 3D hydrogel/stem cell model. Journal of Biomedical Materials Research Part A. 103 (1), pp. 282-288.
AuthorsDong, C., Webb, R., Peng, Q., Tang, J., Forsyth, N., Chen, G. and Haj, A.
Abstract

This study aimed to design a growth factor loaded copolyester of 3-hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx) nanoparticles containing 3D collagen matrix to achieve growth factor sustained release for long-term stimulation of human mesenchymal stem cells (hMSCs) proliferation/differentiation for tissue engineer application. Platelet-derived growth factor-BB (PDGF-BB), which is known to enhance hMSCs proliferation in human serum, was selected as a model growth factor, and biodegradable copolyester of PHBHHx was chosen to be the sustained release vehicle. PDGF-BB phospholipid complex encapsulated PHBHHx nanoparticles were fabricated, and their effect on hMSCs proliferation was investigated via assays of CCK-8 and live-dead staining to cells inoculated in 2D tissue culture plates and 3D collagen gel scaffolds, respectively. The resulting spherical PHBHHx nanoparticles were stable in terms of their mean particle size, polydispersity index and zeta potential before and after lyophilization. In vitro study revealed a sustained release of PDGF-BB with a low burst release. Furthermore, sustained released PDGF-BB was revealed to significantly promote hMSCs proliferation in both cell monolayer and cell seeded 3D collagen scaffolds inoculated in serum-free media. Therefore, the 3D collagen matrices with locally sustained release growth factor nanoparticles hold promise to be used for stem cell tissue engineering.

KeywordsPHB; mesenchymal stem cell; nanoparticles; platelet-derived growth factor; polyhydroxyalkanoates; tissue engineering
Year2014
JournalJournal of Biomedical Materials Research Part A
Journal citation103 (1), pp. 282-288
PublisherWiley-Blackwell
ISSN1549-3296
Official URLhttp://dx.doi.org/10.1002/jbm.a.35149
Publication dates
Online02 Apr 2014
Publication process dates
Deposited26 Mar 2019
Output statusPublished
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