Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture

Journal article


Otto, I., Levato, R., Webb, R., Khan, I., Breugem, C. and Malda, J. 2018. Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture. European Cells & Materials. 35, pp. 132-150. https://doi.org/10.22203/eCM.v035a10
AuthorsOtto, I., Levato, R., Webb, R., Khan, I., Breugem, C. and Malda, J.
Abstract

Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle. Current regenerative medicine strategies utilize chondrocytes from various locations or mesenchymal stromal cells (MSCs). However, the quality of neo-tissues resulting from these cell types is inadequate due to inefficient chondrogenic differentiation and endochondral ossification, respectively. Recently, a subpopulation of stem/progenitor cells has been identified within the auricular cartilage tissue, with similarities to MSCs in terms of proliferative capacity and cell surface biomarkers, but their potential for tissue engineering has not yet been explored.

This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic differentiation. Neocartilage formation was assessed through gene expression profiling, compression testing, biochemical composition and histology. Similar to MSCs and chondrocytes, AuCPCs displayed a marked ability to generate cartilaginous matrix, although, under the applied culture conditions, MSCs outperformed both cartilage-derived cell types in terms of matrix production and mechanical properties. AuCPCs demonstrated upregulated mRNA expression of elastin, low expression of collagen type X and similar levels of proteoglycan production and mechanical properties as compared to chondrocytes. These results underscored the AuCPCs' tissue-specific differentiation potential, making them an interesting cell source for the next generation of elastic cartilage tissue-engineered constructs.

Year2018
JournalEuropean Cells & Materials
Journal citation35, pp. 132-150
PublisherECM Journal.com
ISSN1473-2262
Digital Object Identifier (DOI)https://doi.org/10.22203/eCM.v035a10
Publication dates
Print27 Feb 2018
Publication process dates
Deposited26 Mar 2019
Accepted author manuscript
Output statusPublished
Additional information

This article is distributed in accordance with Creative Commons Attribution Licence (http://creativecommons.org/licenses/by-sa/4.0/).

Permalink -

https://repository.canterbury.ac.uk/item/88z30/progenitor-cells-in-auricular-cartilage-demonstrate-cartilage-forming-capacity-in-3d-hydrogel-culture

Download files

Accepted author manuscript
  • 1
    total views
  • 1
    total downloads
  • 0
    views this month
  • 0
    downloads this month

Export as

Related outputs

Poly (3-hydroxybutyrate-co-3-hydroxyhexanoate)/collagen hybrid scaffolds for tissue engineering applications.
Lomas, A., Webb, R., Han, J., Chen, G., Sun, X., Zhang, Z., El Haj, A. and Forsyth, N. 2013. Poly (3-hydroxybutyrate-co-3-hydroxyhexanoate)/collagen hybrid scaffolds for tissue engineering applications. Tissue Engineering. 19 (8). https://doi.org/10.1089/ten.TEC.2012.0457
The application of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) scaffolds for tendon repair in the rat model
Webb, R., Dale, T., Lomas, A., Zeng, G., Wimpenny, I., El Haj, A., Forsyth, N. and Chen, G. 2013. The application of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) scaffolds for tendon repair in the rat model. Biomaterials. 34 (28), pp. 6683-6694. https://doi.org/10.1016/j.biomaterials.2013.05.041
Sustained PDGF-BB release from PHBHHx loaded nanoparticles in 3D hydrogel/stem cell model
Dong, C., Webb, R., Peng, Q., Tang, J., Forsyth, N., Chen, G. and Haj, A. 2014. Sustained PDGF-BB release from PHBHHx loaded nanoparticles in 3D hydrogel/stem cell model. Journal of Biomedical Materials Research Part A. 103 (1), pp. 282-288.
Controlled production of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) nanoparticles for targeted and sustained drug delivery
Heathman, T., Webb, R., Han, J., Dan, Z., Chen, G., Forsyth, N., El Haj, A., Zhang, Z. and Sun, X. 2014. Controlled production of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) nanoparticles for targeted and sustained drug delivery. European Journal of Pharmaceutical Sciences. 103 (8), pp. 2498-2508. https://doi.org/10.1002/jps.24035
Opportunities for zonal cartilage regeneration: progenitor cell-laden hydrogels and bioprinting
Levato, R., Mouser, V., Webb, R., Otto, I., Gawlitta, D., van Weeren, P., Khan, I. and Malda, J. 2016. Opportunities for zonal cartilage regeneration: progenitor cell-laden hydrogels and bioprinting.
Tenogenic differentiation of human embryonic stem cells
Dale, T., Mazher, S., Webb, R., Zhou, J., Maffulli, N., Chen, G., El Haj, A. and Forsyth, N. 2018. Tenogenic differentiation of human embryonic stem cells. Tissue Engineering. 5-6, pp. 361-368. https://doi.org/10.1089/ten.tea.2017.0017
Macro-porous micro-carriers as a vehicle for chondroprogenitor expansion and scaffold formation for the rapid biofabrication of osteochondral implants
Webb, R., Levato, R., Moshkbouymatin, N., Zhang, Y., Francis, L., van Weeren, P., Malda, J. and Khan, I. 2017. Macro-porous micro-carriers as a vehicle for chondroprogenitor expansion and scaffold formation for the rapid biofabrication of osteochondral implants.
The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells
Levato, R., Webb, R., Otto, I., Mensinga, A., Zhang, Y., van Rijen, M., van Weeren, P., Khan, I. and Malda, J. 2017. The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells. Acta Biomaterialia. 61, pp. 41-53. https://doi.org/10.1016/j.actbio.2017.08.005
Isolation of mesenchymal stem cells from bone marrow aspirate
Markides, H., Webb, W.R., El Haj, A.J., Chippendale, T., Coopman, K., Rafiq, Q. and Hewitt, C. 2019. Isolation of mesenchymal stem cells from bone marrow aspirate. in: Moo-Young, M. (ed.) Comprehensive Biotechnology Elsevier. pp. 137-148
Progenitor cells in auricular cartilage demonstrate promising cartilage regenerative potential in 3D hydrogel culture
Otto, I. A, Levatto, R., Webb, R., Khan I. M., Breugem, C.C. and Malda, J. 2017. Progenitor cells in auricular cartilage demonstrate promising cartilage regenerative potential in 3D hydrogel culture.